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SDS-PAGE Immunoblot Analysis

  • Patricia KlemmerEmail author
Protocol
Part of the Neuromethods book series (NM, volume 57)

Abstract

Immunoblotting is a core method in molecular neuroscience to detect and/or quantify single proteins in a complex mixture. The whole procedure covers the solubilization of proteins, their separation by mass on an SDS-polyacrylamide gel, the electrophoretic transfer to a membrane, and the immunodetection of the target proteins by using specific antibodies. Here we provide protocols for SDS-PAGE followed by electroblotting combined with immunostaining. Useful hints are included for method optimization.

Key words

Western blotting SDS-polyacrylamide gel 

References

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive ResearchVU UniversityAmsterdamThe Netherlands

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