Neuroproteomics: Deciphering Brain Function and Disorders

  • Ka Wan LiEmail author
Part of the Neuromethods book series (NM, volume 57)


Neuroproteomics is a branch of proteomics that specifically studies qualitatively and/or quantitatively the tissue/organelle proteomes of the nervous system. This chapter introduces the various aspects of neuroproteomics, and outlines the range of methods that are commonly employed.

Key words

Proteomics Brain function Brain disorder Methods 


  1. 1.
    Guitart, X., and Nestler, E. J. (1989) Identification of morphine- and cyclic AMP-regulated phosphoproteins (MARPPs) in the locus coeruleus and other regions of rat brain: regulation by acute and chronic morphine, J Neurosci 9, 4371–4387.PubMedGoogle Scholar
  2. 2.
    Castellucci, V. F., Kennedy, T. E., Kandel, E. R., and Goelet, P. (1988) A quantitative analysis of 2-D gels identifies proteins in which labeling is increased following long-term sensitization in Aplysia, Neuron 1, 321–328.PubMedCrossRefGoogle Scholar
  3. 3.
    Choudhary, C., and Mann, M. (2010) Decoding signalling networks by mass spectrometry-based proteomics, Nature reviews 11, 427–439.PubMedCrossRefGoogle Scholar
  4. 4.
    Li, K. W., and Jimenez, C. R. (2008) Synapse proteomics: current status and quantitative applications, Expert review of proteomics 5, 353–360.PubMedCrossRefGoogle Scholar
  5. 5.
    Lull, M. E., Freeman, W. M., VanGuilder, H. D., and Vrana, K. E. (2010) The use of neuroproteomics in drug abuse research, Drug and alcohol dependence 107, 11–22.PubMedCrossRefGoogle Scholar
  6. 6.
    Li, K. W., and Smit, A. B. (2008) Subcellular proteomics in neuroscience, Front Biosci 13, 4416–4425.PubMedCrossRefGoogle Scholar
  7. 7.
    Li, K. W., Klemmer, P., and Smit, A. B. (2010) Interaction proteomics of synapse protein complexes, Analytical and bioanalytical chemistry 397, 3195–3202.PubMedCrossRefGoogle Scholar
  8. 8.
    Westman-Brinkmalm, A., Ruetschi, U., Portelius, E., Andreasson, U., Brinkmalm, G., Karlsson, G., Hansson, S., Zetterberg, H., and Blennow, K. (2009) Proteomics/­peptidomics tools to find CSF biomarkers for neurodegenerative diseases, Front Biosci 14, 1793–1806.PubMedCrossRefGoogle Scholar
  9. 9.
    Kovacech, B., Zilka, N., and Novak, M. (2009) New age of neuroproteomics in Alzheimer’s disease research, Cellular and molecular neurobiology 29, 799–805.PubMedCrossRefGoogle Scholar
  10. 10.
    Bayes, A., and Grant, S. G. (2009) Neuroproteomics: understanding the molecular organization and complexity of the brain, Nat Rev Neurosci 10, 635–646.PubMedCrossRefGoogle Scholar
  11. 11.
    Trinidad, J. C., Thalhammer, A., Specht, C. G., Lynn, A. J., Baker, P. R., Schoepfer, R., and Burlingame, A. L. (2008) Quantitative analysis of synaptic phosphorylation and protein expression, Mol Cell Proteomics 7, 684–696.PubMedGoogle Scholar
  12. 12.
    Van den Oever, M. C., Goriounova, N. A., Li, K. W., Van der Schors, R. C., Binnekade, R., Schoffelmeer, A. N., Mansvelder, H. D., Smit, A. B., Spijker, S., and De Vries, T. J. (2008) Prefrontal cortex AMPA receptor plasticity is crucial for cue-induced relapse to heroin-seeking, Nature neuroscience 11, 1053–1058.PubMedCrossRefGoogle Scholar
  13. 13.
    Li, K. W., Miller, S., Klychnikov, O., Loos, M., Stahl-Zeng, J., Spijker, S., Mayford, M., and Smit, A. B. (2007) Quantitative proteomics and protein network analysis of hippocampal synapses of CaMKIIalpha mutant mice, Journal of proteome research 6, 3127–3133.PubMedCrossRefGoogle Scholar
  14. 14.
    Hilger, M., Bonaldi, T., Gnad, F., and Mann, M. (2009) Systems-wide analysis of a phosphatase knock-down by quantitative proteomics and phosphoproteomics, Mol Cell Proteomics 8, 1908–1920.PubMedCrossRefGoogle Scholar
  15. 15.
    Ye, J., Zhang, X., Young, C., Zhao, X., Hao, Q., Cheng, L., and Jensen, O. N. (2010) Optimized IMAC-IMAC Protocol for Phosphopeptide Recovery from Complex Biological Samples, Journal of proteome research 9, 3561–3573.PubMedCrossRefGoogle Scholar
  16. 16.
    Tsai, C. F., Wang, Y. T., Chen, Y. R., Lai, C. Y., Lin, P. Y., Pan, K. T., Chen, J. Y., Khoo, K. H., and Chen, Y. J. (2008) Immobilized metal ­affinity chromatography revisited: pH/acid control toward high selectivity in phosphoproteomics, Journal of proteome research 7, 4058–4069.PubMedCrossRefGoogle Scholar
  17. 17.
    Martin, S., Nishimune, A., Mellor, J. R., and Henley, J. M. (2007) SUMOylation regulates kainate-receptor-mediated synaptic transmission, Nature 447, 321–325.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive ResearchVU UniversityAmsterdamThe Netherlands

Personalised recommendations