Skip to main content
SpringerLink
Log in

Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye

  • Protocol
  • First Online:
Mammalian Cell Viability

Part of the book series: Methods in Molecular Biology ((MIMB,volume 740))

Abstract

The Alamar Blue assay is based on enzymatic reduction of indicator dye by viable cells and serves as an effective tool for assessing cell proliferation and as a screening technique. It can be applied in studies concentrating on animal, plant, yeast, and bacteria cells. Among the various methods for cell viability and cytotoxicity, it utilises all features of ideal and reliable test; it is one-step, sensitive, safe, non-toxic for cells, and cost-effective.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Twigg R.S. (1945) Oxidation-reduction aspects of resazurin. Nature, 155:401–402

    Article  CAS  Google Scholar 

  2. Nixon M. C. and Lamb A. B. (1945) Resazurin Test for Grading Raw Milk. Can J Comp Med Vet Sci, 9(1):18–23

    PubMed  CAS  Google Scholar 

  3. Ali-Vehmas T., Louhi M., and Sandholm M. (1991) Automation of the resazurin reduction test using fluorometry of microtitration trays. Zentralbl Veterinarmed B, 38(5):358–372

    PubMed  CAS  Google Scholar 

  4. De Jong D. W. and. Woodlief W. G. (1977) Fluorimetric assay of tobacco leaf dehydrogenases with resazurin. Biochim Biophys Acta, 484(2):249–259

    PubMed  Google Scholar 

  5. Glass R. H., Ericsson S. A., Ericsson R. J., Drouin M. T., Marcoux L. J., and Sullivan H. (1991) The resazurin reduction test provides an assessment of sperm activity. Fertil Steril, 56(4):743–746

    PubMed  CAS  Google Scholar 

  6. To W. K., Fothergill A. W., and Rinaldi M. G. (1995) Comparative evaluation of macrodilution and alamar colorimetric microdilution broth methods for antifungal susceptibility testing of yeast isolates. J Clin Microbiol, 33(10):2660–2664

    PubMed  CAS  Google Scholar 

  7. O’Brien J., Wilson I., Orton T., and Pognan F. (2000) Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem, 267(17):5421–5426

    Article  PubMed  Google Scholar 

  8. Perrot S., Dutertre-Catella H., Martin C., Rat P., and Warnet J. M. (2003) Resazurin metabolism assay is a new sensitive alternative test in isolated pig cornea. Toxicol Sci, 72(1):122–129

    Article  PubMed  CAS  Google Scholar 

  9. Hamid R., Rotshteyn Y., Rabadi L., Parikh R., and Bullock P. (2004) Comparison of alamar blue and MTT assays for high through-put screening. Toxicol In Vitro, 18(5):703–710

    Article  PubMed  CAS  Google Scholar 

  10. Larson E. M., Doughman D. J., Gregerson D. S., and Obritsch W. F. (1997) A new, -simple, nonradioactive, nontoxic in vitro assay to monitor corneal endothelial cell viability. Invest Ophthalmol Vis Sci, 38(10):1929–1933

    PubMed  CAS  Google Scholar 

  11. Jonsson K. B., Frost A., Larsson R., Ljunghall S., and Ljunggren O. (1997) A new fluorometric assay for determination of osteoblastic proliferation: effects of glucocorticoids and insulin-like growth factor-I. Calcif Tissue Int, 60(1):30–36

    Article  PubMed  CAS  Google Scholar 

  12. O’Brien, J., Wilson, I., Orton, T., and Pognan, F. (2000) Pognan. Investigation of the Alamar blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur. J. Biochem, 267, 5421–5426.

    Google Scholar 

  13. Gonzalez R. J. and Tarloff J. B. (2001) Evaluation of hepatic subcellular fractions for Alamar blue and MTT reductase activity. Toxicol In Vitro, 15(3):257–259

    Article  PubMed  CAS  Google Scholar 

  14. Nakayama G. R., Caton M. C., Nova M. P., and Parandoosh Z. (1997) Assessment of the Alamar Blue assay for cellular growth and -viability in vitro. J Immunol Methods, 204(2):205–208

    Article  PubMed  CAS  Google Scholar 

  15. De Jong, D. W. and Woodlief, W. G. (1977) Fluorometric assay of tobacco leaf dehydrogenases with resazurin. Biochim. Biophys. Acta, 484, 249–259.

    PubMed  Google Scholar 

  16. Squatrito R. C., Connor J. P., and Buller R. E. (1995) Comparison of a novel redox dye cell growth assay to the ATP bioluminescence assay. Gynecol Oncol, 58(1):101–105

    Article  PubMed  CAS  Google Scholar 

  17. Jonsson, K. B., Frost, A., Larsson, R., Ljunghall, S., and Ljunggren, O. (1997) A new fluorometric assay for determination of osteoblastic proliferation: effects of glucocorticoids and insulin-like growth factor-I. Calcif. Tissue Int, 60, 30–36.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. AO Research Institute, Davos Platz, Switzerland

    Ewa M. Czekanska

Authors
  1. Ewa M. Czekanska
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ewa M. Czekanska .

Editor information

Editors and Affiliations

  1. AO Research Institute, Clavadelerstrasse 8, Davos Platz, 7270, Switzerland

    Martin J. Stoddart

Rights and permissions

Reprints and permissions

Copyright information

© 2011 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Czekanska, E.M. (2011). Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye. In: Stoddart, M. (eds) Mammalian Cell Viability. Methods in Molecular Biology, vol 740. Humana Press. https://doi.org/10.1007/978-1-61779-108-6_5

Download citation

  • DOI: https://doi.org/10.1007/978-1-61779-108-6_5

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-107-9

  • Online ISBN: 978-1-61779-108-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation