Abstract
Bioluminescence from murine stem cells tagged with the luciferase gene reporter and distributed within three-dimensional scaffolds of two different materials is quantified in vitro and in vivo. The luminescence emitted from cells adhering to the scaffolds tested is monitored noninvasively using a bioluminescence imaging system. Monitoring the kinetics of luciferase expression via bioluminescence enables real-time assessment of cell survival and proliferation on scaffolds both in vitro and in vivo over prolonged (8 weeks) periods of time.
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Acknowledgments
The authors gratefully acknowledge Dr. Jiri Honiger (Service de chirurgie orthopédique, Saint-Antoine Hospital, Paris, France) for donating the AN69 polymer scaffolds and Inoteb Inc. (Levallois-Perret, France) for donating the coral scaffolds used in this study. The authors also thank the Plate-Forme d’Imagerie Dynamique at the Pasteur Institute, Paris, France, for their assistance with bioluminescent imaging and Professor Rena Bizios and Dr. Ines Sherifi for critically reading the manuscript. This project was supported by the Centre National de la Recherche Scientifique and by a grant from the “Gueules cassées” Foundation.
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Oudina, K., Cambon-Binder, A., Logeart-Avramoglou, D. (2011). Noninvasive Bioluminescent Quantification of Viable Stem Cells in Engineered Constructs. In: Stoddart, M. (eds) Mammalian Cell Viability. Methods in Molecular Biology, vol 740. Humana Press. https://doi.org/10.1007/978-1-61779-108-6_18
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DOI: https://doi.org/10.1007/978-1-61779-108-6_18
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