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Atomic Force Microscopy Studies on Circular DNA Structural Changes by Vincristine and Aspirin

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Atomic Force Microscopy in Biomedical Research

Part of the book series: Methods in Molecular Biology ((MIMB,volume 736))

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Abstract

In this chapter, we have presented materials and methods to study the interaction between DNA and small molecule drugs by AFM. The detailed AFM imaging of the circular DNA after incubation with ­various concentrations of vincristine and aspirin have been demonstrated. The immobilization of DNA fragments on mica surface as well as the force between tip and sample plays an important role for successful imaging of DNA–drug complexes. How to quantitatively describe the conformations and structures of circular DNA molecules and their changes is also introduced. Our work indicates that the AFM is a powerful tool in studying the interaction between DNA and small molecules.

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Acknowledgments

This work was financially supported by the National Basic Research Program of China (973 Program: 2007CB936300), NSFC (No. 20875014 and 30870626) and 2008DFA51180.

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Authors and Affiliations

  1. State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China

    Zhongdang Xiao

Authors
  1. Zhongdang Xiao
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  2. Lili Cao
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  3. Dan Zhu
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  4. Zuhong Lu
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Corresponding author

Correspondence to Zhongdang Xiao .

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Editors and Affiliations

  1. School of Medicine, Department of Pharmacology, University of Milan, Via Vanvitelli 32, Milano, 20129, Italy

    Pier Carlo Braga

  2. , Robotics, Brain & Cognitive Sciences, Italian Institute of Technology, Via Morego 30, Genova, 16163, Italy

    Davide Ricci

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Xiao, Z., Cao, L., Zhu, D., Lu, Z. (2011). Atomic Force Microscopy Studies on Circular DNA Structural Changes by Vincristine and Aspirin. In: Braga, P., Ricci, D. (eds) Atomic Force Microscopy in Biomedical Research. Methods in Molecular Biology, vol 736. Humana Press. https://doi.org/10.1007/978-1-61779-105-5_26

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  • DOI: https://doi.org/10.1007/978-1-61779-105-5_26

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-104-8

  • Online ISBN: 978-1-61779-105-5

  • eBook Packages: Springer Protocols

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