Abstract
Bacterial infection is a major challenge in biomaterials development. The adhesion of microorganisms to the material surface is the first step in infectious conditions and this quickly leads to the formation of biofilms on a material surface. A unique attribute of atomic force microscopy (AFM) is that it reveals not only the morphology of cells and the surface roughness of the substrate, but it can also quantify the adhesion force between bacteria and surfaces. We have shown that fluoroalkylsilane (FAS) and octadecyltrichlorosilane (OTS)-coated silicone samples exhibit greater potential for reducing E. coli JM 109 adhesion than heparin- and hyaluronan-modified samples. The force curves obtained from AFM can be used as a primary indicator in predicting bacterial adhesion.
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Acknowledgments
The author would like to thank Professors Guangzhao Mao and Gina Shreve, Chemical Engineering and Materials Science Department of Wayne State University, for their help in using the equipment. Financial support of the research by TACOM (contract no. DAAE07-03-C-L140) is gratefully acknowledged.
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Cao, T. et al. (2011). Nanoscale Investigation on E. coli Adhesion to Modified Silicone Surfaces. In: Braga, P., Ricci, D. (eds) Atomic Force Microscopy in Biomedical Research. Methods in Molecular Biology, vol 736. Humana Press. https://doi.org/10.1007/978-1-61779-105-5_22
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DOI: https://doi.org/10.1007/978-1-61779-105-5_22
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