Abstract
Here, we describe the methodology currently used to analyze the structural organization of protozoa of the Trypanosomatidae family by atomic force microscopy. The results are compared with those obtained using light, scanning, and transmission electron microscopy.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Binnig, G. and Rohrer, H. (1982) Scanning tunneling microscopy. Helv. Phys. Acta. 55, 726–735.
Binnig, G., Quate, C. F. and Gerber, C. (1986) Atomic force microscopy. Phys. Rev. Lett. 56, 930–933.
Bustamante, C. and Dunlap, D. (1991) Application of scanning tunneling microscopy to structural biology. Semin. Cell Biol. 2, 179–185.
Dvorak, J. A., Kobayashi, S., Abe, K., Fujiwara, T., Takeuchi, T. and Nagao, E. (2000) The application of the atomic force microscope to studies of medically important protozoan parasites. J. Elec. Microsc. 49, 429–435.
Akaki, M., Nakano, Y., Nagayasu, E., Nagakura, K., Kawai, S. and Aikawa, M. (2001) Invasive forms of Toxoplasma gondii, Leishmania amazonensis and Trypanosoma cruzi have a positive charge at their contact site with host cells. Parasitol. Res. 87, 193–197.
Dufrêne, Y. F. (2002) Atomic force microscopy, a powerful tool in microbiology. J. Bacteriol. 184, 5205–5213.
Dufrêne, Y. F. (2008) Towards nanomicrobiology using atomic force microscopy. Nat. Ver. Microbiol. 6, 674–680.
Wright, C. J. and Armstrong, I. (2006) The application of atomic force microscopy force measurements to the characterization of microbial surfaces. Surf. Interface Anal. 38, 1419–1428.
Garcia, C. R., Takeuschi, M., Yoshioka, K. and Miyamoto, H. (1997) Imaging Plasmodium falciparum-infected ghost and parasite by atomic force microscopy. J. Struct. Biol. 119, 92–98.
Rocha, G. M., Miranda, K., Weissmüller, G., Bisch, P. M., de Souza, W. (2008) Ultrastructure of Trypanosoma cruzi revisited by atomic force microscopy. Microsc. Res. Tech. 71, 133–139.
Acknowledgments
The authors are grateful for support from Conselho Nacional de Desenvolvimento Cinetífico e Tecnológico (CNPq), Financiadora de Estudos e Projetos (FINEP), Fundação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
de Souza, W., Rocha, G.M., Miranda, K., Bisch, P.M., Weissmuller, G. (2011). Atomic Force Microscopy as a Tool for the Study of the Ultrastructure of Trypanosomatid Parasites. In: Braga, P., Ricci, D. (eds) Atomic Force Microscopy in Biomedical Research. Methods in Molecular Biology, vol 736. Humana Press. https://doi.org/10.1007/978-1-61779-105-5_14
Download citation
DOI: https://doi.org/10.1007/978-1-61779-105-5_14
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-104-8
Online ISBN: 978-1-61779-105-5
eBook Packages: Springer Protocols