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Microbial Toxins pp 187–194Cite as

Determination of Fumonisins B1 and B2 in Maize Food Products by a New Analytical Method Based on High-Performance Liquid Chromatography and Fluorimetric Detection with Post-column Derivatization

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 739))

Abstract

A sensitive and selective analytical method for the quantitative determination of fumonisins B1 (FB1) and B2 (FB2) in maize-based foods for direct human consumption is described. The method, based on high-performance liquid chromatography and fluorescence detection, presents a rapid and automated online post-column derivatization, performed with o-phthalaldehyde and N,N-dimethyl-2-mercaptoethylamine (Thiofluor™). A complete separation of fumonisins is achieved in less than 13 min by using a C18 column and a gradient elution. Fumonisins are extracted from the sample with a mixture of water, acetonitrile, and methanol. The filtered extract is purified by immunoaffinity column and FB1 and FB2 are eluted with methanol. The method has been successfully validated, and performances comply with ­criteria of the Regulation EC No 401/2006.

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Acknowledgments

The authors gratefully acknowledge P. D’Antini (Istituto Zoopro­fila­ttico Sperimentale della Puglia e della Basilicata) for the technical assistance. Ministero della Salute (Rome, Italy) is thanked for providing the financial support.

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Authors and Affiliations

  1. Dipartimento di Scienze Agro-Ambientali, Chimica e Difesa Vegetale and BIOAGROMED, Università degli Studi di Foggia, Foggia, Italy

    Diego Centonze

Authors
  1. Marilena Muscarella
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  2. Sonia Lo Magro
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  3. Donatella Nardiello
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  4. Carmen Palermo
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  5. Diego Centonze
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Corresponding author

Correspondence to Diego Centonze .

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Editors and Affiliations

  1. Leibniz-Zentrum fur Medizin und Biowisse, Forschungsinstitut Borstel, Parkallee 4a/c, Borstel, 23845, Germany

    Otto Holst

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Muscarella, M., Magro, S.L., Nardiello, D., Palermo, C., Centonze, D. (2011). Determination of Fumonisins B1 and B2 in Maize Food Products by a New Analytical Method Based on High-Performance Liquid Chromatography and Fluorimetric Detection with Post-column Derivatization. In: Holst, O. (eds) Microbial Toxins. Methods in Molecular Biology, vol 739. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-102-4_16

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  • DOI: https://doi.org/10.1007/978-1-61779-102-4_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-101-7

  • Online ISBN: 978-1-61779-102-4

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