Abstract
Gene transfer of specific growth factors is suitable for inducing chondrogenic differentiation of mesenchymal cells to be used for cartilage regeneration. However, extent and quality of repair tissue formation also depend on biomechanical and metabolic influences that can only be studied in vivo. We describe three methods to evaluate viral gene transfer into mesenchymal cells in animal models of articular cartilage defects, e.g., mouse, rat and miniature pig models, focussing on the repair of hyaline cartilage tissue.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Luyten F. P. DBC, Dell`Accio F. Identification and characterization of human cell populations capable of forming stable hyaline cartilage in vivo. In: Hascall V.C. KKE, editor. The many faces of osteoarthritis. Basel: Birkhaeuser; 2002. p. 67–76.
Gelse K, Muhle C, Franke O, Park J, Jehle M, Durst K, et al. Cell-based resurfacing of large cartilage defects: Long-term evaluation of grafts from autologous transgene-activated periosteal cells in a porcine model of osteoarthritis. Arthritis Rheum 2008;58:475–488.
Robins JC, Akeno N, Mukherjee A, Dalal RR, Aronow BJ, Koopman P, et al. Hypoxia induces chondrocyte-specific gene expression in mesenchymal cells in association with transcriptional activation of Sox9. Bone 2005;37:313–22.
Gelse K, Muhle C, Knaup K, Swoboda B, Wiesener M, Hennig F, et al. Chondrogenic differentiation of growth factor-stimulated precursor cells in cartilage repair tissue is associated with increased HIF-1alpha activity. Osteoarthritis Cartilage 2008;16:1457–65.
Klein-Nulend J, Semeins CM, Mulder JW, Winters HA, Goei SW, Ooms ME, et al. Stimulation of cartilage differentiation by osteogenic protein-1 in cultures of human perichondrium. Tissue Eng 1998;4:305–13.
Wozney JM, Rosen V, Celeste AJ, Mitsock LM, Whitters MJ, Kriz RW, et al. Novel regulators of bone formation: molecular clones and activities. Science 1988;242:1528–34.
Gelse K, Schneider H. Ex vivo gene therapy approaches to cartilage repair. Adv Drug Deliv Rev 2006;58:259–84.
Evans CH, Ghivizzani SC, Robbins PD. Gene therapy for arthritis: what next? Arthritis Rheum 2006;54:1714–29.
Gelse K, Jiang QJ, Aigner T, Ritter T, Wagner K, Poschl E, et al. Fibroblast-mediated delivery of growth factor complementary DNA into mouse joints induces chondrogenesis but avoids the disadvantages of direct viral gene transfer. Arthritis Rheum 2001;44:1943–53.
Gelse K, von der Mark K, Aigner T, Park J, Schneider H. Articular cartilage repair by gene therapy using growth factor-producing mesenchymal cells. Arthritis Rheum 2003;48:430–441.
Gelse K, Pfander D, Obier S, Knaup KX, Wiesener M, Hennig FF, et al. Role of hypoxia-inducible factor 1 alpha in the integrity of articular cartilage in murine knee joints. Arthritis Res Ther 2008;10:R111.
Acknowledgements
This work was supported by the Interdisciplinary Centre for Clinical Research of the University of Erlangen-Nuernberg.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC 2011
About this protocol
Cite this protocol
Gelse, K., Schneider, H. (2011). In Vivo Evaluation of Gene Transfer into Mesenchymal Cells (In View of Cartilage Repair). In: Merten, OW., Al-Rubeai, M. (eds) Viral Vectors for Gene Therapy. Methods in Molecular Biology, vol 737. Humana Press. https://doi.org/10.1007/978-1-61779-095-9_16
Download citation
DOI: https://doi.org/10.1007/978-1-61779-095-9_16
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-094-2
Online ISBN: 978-1-61779-095-9
eBook Packages: Springer Protocols