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Herpes Simplex Virus Type 1-Derived Recombinant and Amplicon Vectors

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Viral Vectors for Gene Therapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 737))

Abstract

Herpes simplex virus type 1 (HSV-1) is a human pathogen whose lifestyle is based on a long-term dual interaction with the infected host, being able to establish both lytic and latent infections. The virus genome is a 153 kbp double-stranded DNA molecule encoding more than 80 genes. The interest of HSV-1 as gene transfer vector stems from its ability to infect many different cell types, both quiescent and proliferating cells, the very high packaging capacity of the virus capsid, the outstanding neurotropic adaptations that this virus has evolved, and the fact that it never integrates into the cellular chromosomes, thus avoiding the risk of insertional mutagenesis. Two types of vectors can be derived from HSV-1, recombinant vectors and amplicon vectors, and different methodologies have been developed to prepare large stocks of each type of vector. This chapter summarizes (1) the two approaches most commonly used to prepare recombinant vectors through homologous recombination, either in eukaryotic cells or in bacteria, and (2) the two methodologies currently used to generate helper-free amplicon vectors, either using a bacterial artificial chromosome (BAC)-based approach or a Cre/loxP site-specific recombination strategy.

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Author information

Authors and Affiliations

  1. Université Lyon 1, Lyon, France

    Alberto L. Epstein

  2. Centre de Génétique et Physiologie Moléculaire et Cellulaire, CNRS, UMR5534, Villeurbanne, France

    Alberto L. Epstein

Authors
  1. Cornel Fraefel
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  2. Peggy Marconi
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  3. Alberto L. Epstein
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Corresponding author

Correspondence to Alberto L. Epstein .

Editor information

Editors and Affiliations

  1. Généthon, rue de l'Internationale 1, Évry CX 2, 91002, France

    Otto-Wilhelm Merten

  2. Chemical & Bioprocess Engineering, University College Dublin, Belfield, Dublin, 4, Ireland

    Mohamed Al-Rubeai

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© 2011 Springer Science+Business Media, LLC 2011

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Fraefel, C., Marconi, P., Epstein, A.L. (2011). Herpes Simplex Virus Type 1-Derived Recombinant and Amplicon Vectors. In: Merten, OW., Al-Rubeai, M. (eds) Viral Vectors for Gene Therapy. Methods in Molecular Biology, vol 737. Humana Press. https://doi.org/10.1007/978-1-61779-095-9_13

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  • DOI: https://doi.org/10.1007/978-1-61779-095-9_13

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-094-2

  • Online ISBN: 978-1-61779-095-9

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