Abstract
The implementation of quantitative approaches in telomere chromosome-oriented FISH (telomeric CO-FISH) allows the assessment of the relative efficiency of lagging versus leading strand telomere replication and thus provides information on the implicated mechanisms. Here, we describe a simple method for telomere strand-specific analyses and discuss its potential applications.
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Acknowledgments
Work in the Londoño laboratory is supported by “La Ligue contre le Cancer,” the “Fondation pour la Recherche Medicale, FRM,” the “Association pour la Recherche sur le Cancer, ARC.” I. O-G. is the recipient of a post-doctoral fellowship from Agence Nationale de la Recherche (ANR) and ARC.
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Ourliac-Garnier, I., Londoño-Vallejo, A. (2011). Telomere Strand-Specific Length Analysis by Fluorescent In Situ Hybridization (Q-CO-FISH). In: Songyang, Z. (eds) Telomeres and Telomerase. Methods in Molecular Biology, vol 735. Humana Press. https://doi.org/10.1007/978-1-61779-092-8_4
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DOI: https://doi.org/10.1007/978-1-61779-092-8_4
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