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Mimicry Technology: Suppressing Small RNA Activity in Plants

  • Ignacio Rubio-SomozaEmail author
  • Pablo Andrés Manavella
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 732)

Abstract

Small RNA suppression constitutes one of the major difficulties for a full molecular characterization of their specific roles in plants. Taking advantage of the latest insights into the new post-biogenesis layer of regulation in microRNA (miRNA) activity, it is possible to overcome the above-mentioned limitation (Nat Genet 39:1033–1037, 2007). We engineered the IPS1 non-coding RNA to bear a complementary sequence to a given miRNA family, resulting in specific sequestration of RISC complexes. MIMIC technology allows for the constitutive release of all of the potential targets of a miRNA family as well as tissue-specific and inducible suppression of its activity.

Key words

Target mimicry microRNA Small RNA suppression IPS1 

Notes

Acknowledgments

The authors thank Prof. Detlef Weigel and the microRNA team at his lab for continuous and helpful discussion and Beth Rowans for manuscript comments and text editing. Authors are supported by European Community FP6 IP SIROCCO (contract LSHG-CT-2006-037900) and by the Max Planck Society.

References

  1. 1.
    Franco-Zorrilla, J.M., Valli, A., Todesco, M., Mateos, I., Puga, M.I., Rubio-Somoza, I., et al. (2007) Target mimicry provides a new mechanism for regulation of microRNA ­activity. Nat Genet 39, 1033–1037.PubMedCrossRefGoogle Scholar
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    Brand, U., Grunewald, M., and Simon, R. (2002) ­regulation of CLV3 expression by two homeobox­ genes in arabidopsis. Plant Phys 129, 565–575.CrossRefGoogle Scholar
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    Weigel, D., and Glazebrook, J. (2002) Arabidopsis: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. 354 pGoogle Scholar

Copyright information

© Humana Press 2011

Authors and Affiliations

  1. 1.Max Planck Institute for Developmental BiologyTübingenGermany

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