Abstract
Cell cultures are widely used in biomedical research. Primary cultures are directly obtained from fresh tissue and reproduce during days or weeks the major characteristics of the original tissue cells. Primary cell culture systems have shown their usefulness for studying the specific activities and the underlying mechanisms of a variety of test compounds. Brain primary cultures have become a powerful tool to analyze the toxic effects and mechanisms of potentially harmful agents in the different brain cell types.
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References
Patel AJ, Lewis PD (1988) Brain cell acquisition and neurotropic drugs with special reference to functional teratogenesis. Prog Brain Res 73:389–403
Sanfeliu C, Cristòfol R, Torán N, Rodríguez-Farré E, Kim SU (1999) Use of human central nervous system cell cultures in neurotoxicity testing. Toxicol In Vitro 13:753–759
De Vera N, Martínez E, Sanfeliu C (2008) Spermine induces cell death in cultured human embryonic cerebral cortical neurons. J Neurosci Res 86:861–872
Hertz E, Yu ACH, Hertz L, Juurlink BHJ, Schousboe A (1989) Preparation of primary cultures of mouse cortical neurons. In: Shahar A, de Vellis J, Vernadakis A, Haber B (eds) A dissection and tissue culture manual of the nervous system. Alan R. Liss, New York, pp 203–206
Schousboe A, Meier E, Drejer J, Hertz L (1989) Preparation of primary cultures of mouse (rat) cerebellar granule cells. In: Shahar A, de Vellis J, Vernadakis A, Haber B (eds) A dissection and tissue culture manual of the nervous system. Alan R. Liss, New York, pp 203–206
Giulian D, Baker TJ (1986) Characterization of ameboid microglia isolated from developing mammalian brain. J Neurosci 6:2163–2178
Saura J, Tusell JM, Serratosa J (2003) High-yield isolation of murine microglia by mild trypsinization. Glia 44:183–189
Kim SU (1990) Neurobiology of human oligodendrocytes in culture. J Neurosci Res 27:712–728
Satoh J, Kim SU (1994) HSP72 induction by heat stress in human neurons and glial cells in culture. Brain Res 653:243–250
Satoh J, Kim SU (1994) Proliferation and differentiation of fetal human oligodendrocytes in culture. J Neurosci Res 39:260–272
Freshney RI (1992) Design and layout of the laboratory. In: Freshney RI (ed) Culture of animal cells, 3rd edn. Wiley-Lis, New York, pp 17–30
Hamby ME, Uliasz TF, Hewett SJ, Hewett JA (2006) Characterization of an improved procedure for the removal of microglia from confluent monolayers of primary astrocytes. J Neurosci Methods 150:128–137
Du F, Qian ZM, Zhu L, Wu XM, Qian C, Chan R, Ke Y (2010) Purity, cell viability, expression of GFAP and bystin in astrocytes cultured by different procedures. J Cell Biochem 109:30–37
Acknowledgments
The authors acknowledge support from the following grants: PI06/1212, PI08/1396, PI10/0453 and RD06/0013/1004 from ISCIII, SAF2006-13092-C02-02 and SAF2009-13093-C02-02 from MICINN, Spain, and LSHB-CT-2004-512051 from European Commission.
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Solà, C., Cristòfol, R., Suñol, C., Sanfeliu, C. (2011). Primary Cultures for Neurotoxicity Testing. In: Aschner, M., Suñol, C., Bal-Price, A. (eds) Cell Culture Techniques. Neuromethods, vol 56. Humana Press. https://doi.org/10.1007/978-1-61779-077-5_4
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DOI: https://doi.org/10.1007/978-1-61779-077-5_4
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