Abstract
Cytogenetic analysis is an integral part of the diagnostic work-up of the patient with acute myeloid leukaemia. Conventional cytogenetic analysis relies on obtaining a good quality bone marrow specimen in a timely fashion and setting up at least two short-term cultures. A 15–24-h culture and a 48-h synchronised culture are routinely set up but as the cytogenetics result is often required urgently to determine the type of therapy to be administered, analysis is undertaken using the overnight culture in the first instance. Rapid and accurate analysis relies on obtaining high-quality G-banding. Knowledge of the conditions affecting banding is therefore essential.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Swerdlow, S.H., Campo, E., Harris, N.L., Jaffe, E.S., Pileri, S.A., Stein, H., Thiele, J., Vardiman, J.W. (eds.) (2008). WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. IARC: Lyon.
Grimwade, D., Walker, H., Oliver, F., et al. (1998) The importance of diagnostic cytogenetics on outcome in AML: analysis of 1,612 patients entered into the MRC AML 10 trial. Blood 92, 2322–33.
Slovak, M.L., Kopecky, K.J., Cassileth, P.A., et al. (2000) Karyotypic analysis predicts outcome of preremission and postremission therapy in adult acute myeloid leukemia: a Southwest Oncology Group/Eastern Cooperative Oncology Group study. Blood 96, 4075–83.
Döhner, H., Estey, E.H., Amadori, S., et al. (2010) Diagnosis and management of acute myeloid leukemia in adults: recommendations from an international expert panel, on behalf of the European LeukemiaNet. Blood 115, 453–74.
Webber, L.M. & Garson, O.M. (1983) Fluorodeoxyuridine synchronization of bone marrow cultures. Cancer Genetics & Cytogenetics 8, 123–32.
Barch, M.J., Knutsen, T. & Spurbeck, J.L. (1997) The AGT Cytogenetics Laboratory Manual, 3rd ed. Philadelphia: Lippincott-Raven Publishers.
Swansbury, J. (ed.) (2003). Cancer Cytogenetics Methods and Protocols. Clifton: Humana Press, 83–91.
Rooney, D.E. (2001) Human Cytogenetics: Malignancy and acquired abnormalities, 3rd ed. London: Oxford University Press.
Henegariu, O., Heerema, N.A., Lowe Wright, L. et al. (2001) Improvements in cytogenetic slide preparation: controlled chromosome spreading, chemical aging and gradual denaturing. Cytometry 43, 101–9.
ISCN (2009). An International System for Human Cytogenetic Nomenclature. L.G. Shaffer, M.L. Slovak, L.J. Campbell (eds.). Basel S.: Karger.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Campbell, L.J., White, J.S. (2011). Cytogenetic Analysis in Acute Myeloid Leukaemia. In: Campbell, L. (eds) Cancer Cytogenetics. Methods in Molecular Biology, vol 730. Humana Press. https://doi.org/10.1007/978-1-61779-074-4_5
Download citation
DOI: https://doi.org/10.1007/978-1-61779-074-4_5
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-073-7
Online ISBN: 978-1-61779-074-4
eBook Packages: Springer Protocols