Abstract
A robust procedure for performing fluorescence in situ hybridization (FISH) is described, with tips for troubleshooting. FISH probes are now more reliable and there is a greater range commercially available. FISH is an essential part of the cytogeneticist’s repertoire. It remains a powerful, complementary adjunct to karyotype studies, and knowledge of the underlying chromosome abnormalities can be essential for understanding the FISH signal patterns.
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Swansbury, J., Min, T., Aruliah, S. (2011). Fluorescence In Situ Hybridization Methods and Troubleshooting Applied to Fixed Cell Suspensions. In: Campbell, L. (eds) Cancer Cytogenetics. Methods in Molecular Biology, vol 730. Humana Press. https://doi.org/10.1007/978-1-61779-074-4_3
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DOI: https://doi.org/10.1007/978-1-61779-074-4_3
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