Abstract
Although in situ hybridization has been in use for over 30 years, its application to the study of solid tissue has only recently been adopted. Despite the numerous reports of the viability of formalin-fixed, paraffin-embedded (FFPE) tissue for fluorescence in situ hybridization (FISH) testing, this technique has not been universally implemented in the routine diagnostic setting. This is most likely due to the perception that the process is more technically demanding than FISH using conventional cytogenetic samples. FFPE FISH does, however, enable retrospective analysis of archived tissue samples and is helpful in the diagnosis of morphologically difficult cases such as Burkitt-like lymphoma, diffuse large B-cell lymphoma, and mantle-cell lymphoma.
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References
Schurter MJ, LeBrun DP, Harrison KJ. Improved technique for fluorescence in situ hybridisation analysis of isolated nuclei from archival, B5 or formalin fixed, paraffin wax embedded tissue. Mol Pathol. 2002;55(2): 121–4.
Schofield DE, Fletcher JA. Trisomy 12 in pediatric granulosa-stromal cell tumors. Demonstration by a modified method of fluorescence in situ hybridization on paraffin-embedded material. Am J Pathol. 1992;141(6): 1265–9.
Wolman SR, Waldman FM, Balazs M. Complementarity of interphase and metaphase chromosome analysis in human renal tumours. Genes Chromosomes Cancer. 1993; 6(1):17–23.
Ventura RA, Martin-Subero JI, Jones M, et al. FISH analysis for the detection of lymphoma-associated chromosomal abnormalities in routine paraffin-embedded tissue. J Mol Diagn. 2006;8(2):141–51.
Squire JA, Marrano P, Kolomietz E. FISH in Clinical Cytogenetics. In: Beatty BG, Mai S, Squire JA, eds. FISH A Practical Approach. Oxford University Press, New York, 2002. 183–204.
Roche Applied Science. DIG Application Manual for Nonradioactive In Situ Hybridisation. 3rd Edition. Roche Applied Science, Penzberg. 2002.
Hyytinen E, Visakorpi T, Kallioniemi A, Kallioniemi OP, Isola JJ. Improved technique for analysis of formalin-fixed, paraffin-embedded tumours by fluorescence in situ hybridisation. Cytometry. 1994;16(2):93–9.
Dako Cytomation. Histology FISH Accessory Kit Instructions. 2004. 1–16 (Product Insert).
Schofield DE, Weremowicz S. Current Protocols in Human Genetics. Wiley, New Jersey, USA. 2008;8.8.1–8.8.8.
Hilz H, Wiegers U, Adamietz P. Stimulation of proteinase K action by denaturing agents: application to the isolation of nucleic acids and the degradation of ‘masked’ proteins. Eur J Biochem. 1975;56(1):103–8.
Roche Applied Science. Proteinase K, recombinant, PCR Grade Lot-specific Data Sheet. 2005 (Product Insert).
Cogliatti SB, Novak U, Henz S, et al. Diagnosis of Burkitt lymphoma in due time: a practical approach. Br J Haematol. 2006;134(3):294–301.
Barry TS, Hwang HC, Goldstein LC, Yaziji H, Hing AW, Gown AM. High Sensitivity and Specificity of FISH in Routine Detection of t(14;18)(q32;q21) and t(11;14)(q13;q32) in Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue sections Using Automated Morphometric Image Analysis. United States & Canadian Academy of Pathology 2005 Annual Meeting, Poster 195.
Acknowledgments
I would like to thank Kathy Somana, Kathleen Rayeroux, Bruce Mercer, Crisoula Batzios, and Lynda Campbell for their assistance in the development and implementation of this technique in our laboratory.
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Zordan, A. (2011). Fluorescence In Situ Hybridization on Formalin-Fixed, Paraffin-Embedded Tissue Sections. In: Campbell, L. (eds) Cancer Cytogenetics. Methods in Molecular Biology, vol 730. Humana Press. https://doi.org/10.1007/978-1-61779-074-4_14
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DOI: https://doi.org/10.1007/978-1-61779-074-4_14
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