Abstract
Measurement of multiple analytes can provide increased sensitivity and specificity for the detection and management of disease. The enzyme-linked immunosorbent assay (ELISA) is currently the “gold standard” for protein quantification; however, individual assays for each analyte must be performed, placing demand on sample volume. On the contrary, multiplex assays using microsphere-based technologies allow for multiple analytes to be simultaneously assayed within a single sample. Here, we present a protocol for the preparation and development of a multiple-analyte assay in human plasma using the BioPlex 200 platform (Bio-Rad), which incorporates xMAP technology (Luminex).
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Acknowledgements
We acknowledge the support from the National Health and Medical Research Council (NH & MRC) through Program Grant #487922, and a Peter Doherty Fellowship to O.K.B. (#381411).
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Bernhard, O.K., Mathias, R.A., Barnes, T.W., Simpson, R.J. (2011). A Fluorescent Microsphere-Based Method for Assay of Multiple Analytes in Plasma. In: Simpson, R., Greening, D. (eds) Serum/Plasma Proteomics. Methods in Molecular Biology, vol 728. Humana Press. https://doi.org/10.1007/978-1-61779-068-3_12
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DOI: https://doi.org/10.1007/978-1-61779-068-3_12
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Publisher Name: Humana Press
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Online ISBN: 978-1-61779-068-3
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