Abstract
Full-length complementary DNAs (cDNAs) are an essential resource for functional genomics. Recently, we have developed a simple and efficient method for preparing a full-length cDNA library from a small amount of total RNA, named the “vector-capping” method. The biggest advantage of this method is that the intactness of the cDNA can be assured by the presence of dG at the 5′ end of the full-length cDNA. Furthermore, the cDNA library represents the mRNA population in the cell owing to a bias-free procedure. In this chapter, we describe not only the protocol for preparing the library but also the points for analyzing the 5′-end sequence of the obtained cDNA.
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This work was supported by a grant from the Ministry of Health, Labor, and Welfare of Japan.
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Kato, S., Oshikawa, M., Ohtoko, K. (2011). Full-Length Transcriptome Analysis Using a Bias-Free cDNA Library Prepared with the Vector-Capping Method. In: Lu, C., Browse, J., Wallis, J. (eds) cDNA Libraries. Methods in Molecular Biology, vol 729. Humana Press. https://doi.org/10.1007/978-1-61779-065-2_4
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DOI: https://doi.org/10.1007/978-1-61779-065-2_4
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