Abstract
Applications in a variety of fields rely on the high-throughput ultrasensitive and multiplexed detection of oligonucleotides. However, the conventional microarray-based techniques that employ fluorescent dyes are hampered by several limitations; they require target amplification, fluorophore labeling, and complicated instrumentation, while the fluorophore-labeled species themselves exhibit slow binding kinetics, photo-bleaching effects, and overlapping spectral profiles. Among the emerging nanomaterials that are being used to solve these problems, oligonucleotide–gold nanoparticle conjugates (Oligo-AuNPs) have recently been highlighted due to their unique chemical and physical properties. In this chapter, a detection scheme for oligonucleotides that utilize Oligo-AuNPs is evaluated with multiple oligonucleotide targets. This scheme takes advantage of the sharp melting transitions, intense optical properties, catalytic properties, enhanced binding properties, and the programmable assembly/disassembly of Oligo-AuNPs.
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References
Vlahou, A. and Fountoulakis, A. (2005) Proteomic approaches in the search for disease biomarkers. J. Chromatogr. B 814, 11–19.
Rigaut, G., Shevchenko, A., Rutz, B., Wilm, M., Mann, M., and Seraphin, B. (1999) A generic protein purification method for protein complex characterization and proteome exploration. Nat. Biotechnol. 17, 1030–1032.
Seubert, P., Vigopelfrey, C., Esch, F., Lee, M., Dovey, H., Davis, D., et al. (1992) Isolation and quantification of soluble Alzheimers beta-peptide from biological-fluids. Nature 359, 325–327.
DeRisi, J. L., Iyer, V. R., and Brown, P. O. (1997) Exploring the metabolic and genetic control of gene expression on a genomic scale. Science 278, 680–686.
Rosi, N. L. and Mirkin, C. A. (2005) Nanostructures in biodiagnostics. Chem. Rev. 105, 1547–1562.
Wang, H., Yang, R., Yang, L., and Tan, W. (2009) Nucleic acid conjugated nanomaterials for enhanced molecular recognition. ACS Nano 3, 2451–2460.
Niemeyer, C. M. and Simon, U. (2005) DNA-based assembly of metal nanoparticles. Eur. J. Inorg. Chem. 18, 3641–3655.
Mirkin, C. A., Letsinger, R. L., Mucic, R. C., and Storhoff, J. J. (1996) A DNA-based method for rationally assembling nanoparticles into macroscopic materials. Nature 382, 607–609.
Taton, T. A., Mirkin, C. A., and Letsinger, R. L. (2000) Scanometric DNA array detection with nanoparticle probes. Science 289, 1757–1760.
Anderson, E. S., Dong, M., Morten, M. N., Kasper, J., Subramani, R., Mamdouh, W., et al. (2009) Self-assembly of a nanoscale DNA box with a controllable lid. Nature 459, 73–76.
Alivisatos, A. P., Johnsson, K. P., Peng, X., Wilson, T. E., Loweth, C. J., Bruchez, M. P., et al. (1996) Organization of “nanocrystal molecules” using DNA. Nature 382, 609–611.
Grabar, K. C., Freeman, R. G., Hommer, M. B., and Natan, M. J. (1995) Preparation and characterization of Au colloid monolayers. Anal. Chem. 67, 735–743.
Frens, G. (1973) Controlled nucleation for regulation of particle-size in monodisperse gold suspensions. Nat. Phys. Sci. 241, 20–22.
Daniel, M. C. and Astruc, D. (2004) Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chem. Rev. 104, 293–346.
Jin, R., Wu, G., Li, Z., Mirkin, C. A., and Schatz, G. C. (2003) What controls the melting properties of DNA-linked gold nanoparticle assemblies? J. Am. Chem. Soc. 125, 1643–1654.
Storhoff, J. J., Lazarides, A. A., Mucic, R. C., Mirkin, C. A., Letsinger, R. L., and Schatz, G. C. (2000) What controls the optical properties of DNA-linked gold nanoparticle assemblies? J. Am. Chem. Soc. 122, 4640–4650.
Lee, J.-S., Stoeva, S. I., and Mirkin, C. A. (2006) DNA-induced size-selective separation of mixtures of gold nanoaparticles. J. Am. Chem. Soc. 128, 8899–8903.
Lytton-Jean, A. K. R. and Mirkin, C. A. (2005) A thermodynamic investigation into the binding properties of DNA functionalized gold nanoparticle probes and molecular fluorophore probes. J. Am. Chem. Soc. 127, 12754–12755.
Rosi, N. L., Giljohann, D. A., Thaxton, C. S., Lytton-Jean, A. K. R., Han, M. S., and Mirkin, C. A. (2006) Oligonucleotide-modified gold nanoparticles for intracellular gene regulation. Science 312, 1027–1030.
Letsinger, R. L., Elghanian, R., Viswanadham, G., and Mirkin, C. A. (2000) Use of a steroid cyclic disulfide anchor in constructing gold nanoparticle-oligonucleotide conjugates. Bioconjug. Chem. 11, 289–291.
Li, Z., Jin, R. C., Mirkin, C. A., and Letsinger, R. L. (2002) Multiple thiol-anchor capped DNA-gold nanoparticle conjugates. Nucleic Acids Res. 30, 1558–1562.
Hurst, S. J., Lytton-Jean, A. K. R., and Mirkin, C. A. (2006) Maximizing DNA loading on a range of gold nanoparticle sizes. Anal. Chem. 78, 8313–8318.
Stoeva, S. I., Lee, J.-S., Thaxton, C. S., and Mirkin, C. A. (2006) Multiplexed DNA detection with bio-barcoded nanoparticle probes. Angew. Chem. Int. Ed. 45, 3303–3306.
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Lee, JS. (2011). Multiplexed Detection of Oligonucleotides with Biobarcoded Gold Nanoparticle Probes. In: Hurst, S. (eds) Biomedical Nanotechnology. Methods in Molecular Biology, vol 726. Humana Press. https://doi.org/10.1007/978-1-61779-052-2_2
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DOI: https://doi.org/10.1007/978-1-61779-052-2_2
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