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Electrospun Nanofibrous Scaffolds for Engineering Soft Connective Tissues

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Biomedical Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 726))

Abstract

Tissue-engineered medical implants, such as polymeric nanofiber scaffolds, are potential alternatives to autografts and allografts, which are short in supply and carry risks of disease transmission. These scaffolds have been used to engineer various soft connective tissues such as skin, ligament, muscle, and tendon, as well as vascular and neural tissue. Bioactive versions of these materials have been produced by encapsulating molecules such as drugs and growth factors during fabrication. The fibers comprising these scaffolds can be designed to match the structure of the native extracellular matrix (ECM) closely by mimicking the dimensions of the collagen fiber bundles evident in soft connective tissues. These nanostructured implants show improved biological performance over the bulk materials in aspects of cellular infiltration and in vivo integration, and the topography of such scaffolds has been shown to dictate cellular attachment, migration, proliferation, and differentiation, which are critical steps in engineering complex functional tissues and crucial to improved biocompatibility and functional performance. Nanofiber matrices can be fabricated using a variety of techniques, including drawing, molecular self-assembly, freeze-drying, phase separation, and electrospinning. Among these processes, electrospinning has emerged as a simple, elegant, scalable, continuous, and reproducible technique to produce polymeric nanofiber matrices from solutions and their melts. We have shown the ability of this technique to be used to fabricate matrices composed of fibers from a few hundred nanometers to several microns in diameter by simply altering the polymer solution concentration. This chapter will discuss the use of the electrospinning technique in the fabrication of ECM-mimicking scaffolds. Furthermore, selected scaffolds will be seeded with primary adipose-derived stromal cells, imaged using scanning electron microscopy and confocal microscopy, and evaluated in terms of their capacity toward supporting cellular proliferation over time.

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References

  1. Almarza, A. J., Yang, G., Woo, S. L., Nguyen, T., and Abramowitch, S. D. (2008) Positive Changes in Bone Marrow-Derived Cells in Response to Culture on an Aligned Bioscaffold. Tissue Eng. Part A 14, 1489–1495.

    Article  CAS  Google Scholar 

  2. Li, S., Patel, S., Hashi, C., Huang, N. F., and Kurpinski, K. (2007) Biomimetic Scaffolds made from Biodegradable Nanofibers Covalently Linked with Extracellular Matrix and/or Animal Growth Factors and Entrapped Cells. PCT Designated States: Designated States W: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, K (TRUNCATED).

    Google Scholar 

  3. Laurencin, C. T., Nair, L. S., Bhattacharyya, S., Allcock, H. R., Bender, J. D., Brown, P. W., and Greish, Y. E. (2005) Polymeric Nanofibers for Tissue Engineering and Drug Delivery. Main IPC: A61L027-18; Secondary IPC: A61L027-50, PCT Designated States: Designated States W: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, L (TRUNCATED).

    Google Scholar 

  4. James, R., Kesturu, G., Balian, G., and Chhabra, A. B. (2008) Tendon: Biology, Biomechanics, Repair, Growth Factors, and Evolving Treatment Options. J. Hand Surg. [Am] 33, 102–112.

    Article  Google Scholar 

  5. Kumbar, S. G., James, R., Nukavarapu, S. P., and Laurencin, C. T. (2008) Electrospun Nanofiber Scaffolds: Engineering Soft Tissues. Biomed. Mater. 3, 034002.

    Article  CAS  Google Scholar 

  6. Kumbar, S. G., Nair, L. S., Bhattacharyya, S., and Laurencin, C. T. (2006) Polymeric Nanofibers as Novel Carriers for the Delivery of Therapeutic Molecules. J. Nanosci. Nanotechnol. 6, 2591–2607.

    Article  CAS  Google Scholar 

  7. Kumbar, S. G., Nukavarapu, S. P., James, R., Hogan, M. V., and Laurencin, C. T. (2008) Recent Patents on Electrospun Biomedical Nanostructures: An Overview. Recent Pat. Biomed. Eng. 1, 68–78.

    CAS  Google Scholar 

  8. He, W., Yong, T., Teo, W. E., Ma, Z., and Ramakrishna, S. (2005) Fabrication and Endothelialization of Collagen-Blended Biodegradable Polymer Nanofibers: Potential Vascular Graft for Blood Vessel Tissue Engineering. Tissue Eng. 11, 1574–1588.

    Article  CAS  Google Scholar 

  9. Janjanin, S., Li, W. J., Morgan, M. T., Shanti, R. M., and Tuan, R. S. (2008) Mold-Shaped, Nanofiber Scaffold-Based Cartilage Engineering using Human Mesenchymal Stem Cells and Bioreactor. J. Surg. Res. 149, 47–56.

    Article  CAS  Google Scholar 

  10. Koh, H. S., Yong, T., Chan, C. K., and Ramakrishna, S. (2008) Enhancement of Neurite Outgrowth using Nano-Structured Scaffolds Coupled with Laminin. Biomaterials 29, 3574–3582.

    Article  CAS  Google Scholar 

  11. Park, Y. and Choo, J. E. (2007) Self-Assembly Nanocomposites Comprising Hydrophilic Bioactive Peptides and Hydrophobic Materials for Surface Treatment of Biomaterials and Tissue Regeneration Therapy. PCT Designated States: Designated States W: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KZ, L (TRUNCATED).

    Google Scholar 

  12. Li, X. T., Zhang, Y., and Chen, G. Q. (2008) Nanofibrous Polyhydroxyalkanoate Matrices as Cell Growth Supporting Materials. Biomaterials 29, 3720–3728.

    Article  CAS  Google Scholar 

  13. Stupp, S. I., Hartgerink, J. D., and Niece, K. L. (2004) Self-Assembling Peptide-Amphiphiles and Self-Assembled Peptide Nanofiber Networks for Tissue Engineering. Main IPC: C07K, PCT Designated States: Designated States W: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, L (TRUNCATED).

    Google Scholar 

  14. Liu, X. and Ma, P. X. (2009) Phase Separation, Pore Structure, and Properties of Nanofi-brous Gelatin Scaffolds. Biomaterials 30, 4094–4103.

    Article  CAS  Google Scholar 

  15. Jiang, H., Hu, Y., Li, Y., Zhao, P., and Zhu, K. (2005) Preparation of Core-Shell Nano/Micro-fiber or Capsule for Drug Sustained Release. Main IPC: A61K009-00, Patent Application Country: Application: CN; Patent Country: CN.

    Google Scholar 

  16. Andersen, E., Smith, D., and Reneker, D. (2005) A Medical Device with Nanofiber Outer Surface Layer Incorporating Nitric Oxide and Poly(Ethylenimine) Diazeniumdiolate for Insertion in to Vascular System. Main IPC: A61L029-00, PCT Designated States: Designated States W: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, L (TRUNCATED).

    Google Scholar 

  17. Kumbar, S. G., Bhattacharyya, S., Sethuraman, S., and Laurencin, C. T. (2007) A Preliminary Report on a Novel Electrospray Technique for Nanoparticle Based Biomedical Implants Coating: Precision Electrospraying. J. Biomed. Mater. Res. B 81, 91–103.

    Google Scholar 

  18. Li, W. J., Jiang, Y. J., and Tuan, R. S. (2006) Chondrocyte Phenotype in Engineered Fibrous Matrix Is Regulated by Fiber Size. Tissue Eng. 12, 1775–1785.

    Article  CAS  Google Scholar 

  19. Kumbar, S. G., Nukavarapu, S. P., James, R., Nair, L. S., and Laurencin, C. T. (2008) Electrospun Poly(Lactic Acid-Co-Glycolic Acid) Scaffolds for Skin Tissue Engineering. Biomaterials 29, 4100–4107.

    Article  CAS  Google Scholar 

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Acknowledgments

The authors gratefully acknowledge funding from the NIH (R01 EB004051 and R01 AR052536), the NSF-(EFRI-0736002), and the Coulter Foundation (526203-FY10-01).

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, Department of Chemical, Materials and Biomolecular Engineering, University of Connecticut Health Center, Farmington, CT, USA

    Sangamesh G. Kumbar

Authors
  1. Roshan James
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  2. Udaya S. Toti
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  3. Cato T. Laurencin
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  4. Sangamesh G. Kumbar
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Corresponding author

Correspondence to Sangamesh G. Kumbar .

Editor information

Editors and Affiliations

  1. Center for Nanoscale Materials, Argonne National Laboratory, S. Cass Avenue 9700, Argonne, 60439, Illinois, USA

    Sarah J. Hurst

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James, R., Toti, U.S., Laurencin, C.T., Kumbar, S.G. (2011). Electrospun Nanofibrous Scaffolds for Engineering Soft Connective Tissues. In: Hurst, S. (eds) Biomedical Nanotechnology. Methods in Molecular Biology, vol 726. Humana Press. https://doi.org/10.1007/978-1-61779-052-2_16

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  • DOI: https://doi.org/10.1007/978-1-61779-052-2_16

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-051-5

  • Online ISBN: 978-1-61779-052-2

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