Abstract
RNA interference (RNAi) is a widely used tool for the analysis of gene expression. In this process, short-interfering RNAs (siRNAs) guide the RNA-induced silencing complex (RISC) to complementary target RNA molecules, which are sequence-specifically cleaved by the RISC. In vitro cleavage assays have proved to be powerful tools for the characterization of the RNAi pathway in many different organisms. Therefore, this chapter provides a detailed protocol for in vitro RISC assays.
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Acknowledgments
Our research is supported by the Max-Planck Society, Regensburg University and by grants from the Deutsche Forschungsgemeinschaft (DFG), the European Union (FP6, ERC), the German ministry for education and science (BMBF), and the Bavarian Genome research network (BayGene).
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Stoehr, J., Meister, G. (2011). In Vitro RISC Cleavage Assay. In: Hobman, T., Duchaine, T. (eds) Argonaute Proteins. Methods in Molecular Biology, vol 725. Humana Press. https://doi.org/10.1007/978-1-61779-046-1_6
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DOI: https://doi.org/10.1007/978-1-61779-046-1_6
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