Abstract
The cell surface is covered with a myriad of carbohydrates that form a complex matrix of oligosaccharides. Carbohydrate recognition plays critical roles in pathogenesis, trafficking, and differentiation. Lectin microarray technology presents a novel platform for the high-throughput analysis of these structurally diverse biopolymers. One drawback of this technology has been limitations imposed by the commercially available plant lectins used in the array. Not only are a majority of these plant-derived proteins glycosylated, which can complicate glycomic analysis, but they also differ in activity and availability. Our lab has recently introduced recombinant lectins to enhance the stability and scope of our lectin panel. Herein, we provide a detailed procedure for the expression of bacterially-derived lectins and their application to a recombinant lectin microarray.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Mahal LK (2008) Glycomics: towards bioinformatic approaches to understanding glycosylation. Anticancer Agents Med Chem 8: 37–51
Hirabayashi J (2008) Concept, strategy and realization of lectin-based glycan profiling. J Biochem 144(2):139–147
Pilobello KT, Krishnamoorthy L, Slawek D, Mahal LK (2005) Development of a lectin microarray for the rapid analysis of protein glycopatterns. Chembiochem 6:985–9
Hirabayashi J (2004) Lectin-based structural glycomics: glycoproteomics and glycan profiling. Glycoconj J 21(1):35–40
Sharon N (2006) Carbohydrates as future anti-adhesion drugs for infectious diseases. Biochim Biophys Acta 1760:527–37
Dodson KW, Pinker JS, Rose T, Magnusson G, Hultgren SJ, Waksman G (2001) Structural basis on the interaction of the pyelonephritic E. coli adhesion to its human kidney receptor. Cell 105:733–43
Hsu KL, Gildersleeve JC, Mahal LK (2008) A simple strategy for the creation of a recombinant lectin microarray. Mol Biosyst 4:654–62
Pilobello KT, Slawek DE, Mahal LK (2007) A ratiometric lectin microarray approach to analysis of the dynamic mammalian glycome. Proc Natl Acad Sci USA 104:11534–9
Krishnamoorthy L, Bess JW Jr, Preston AB, Nagashima K, Mahal LK (2009) HIV-1 and microvesicles from T-cells share a common glycome, arguing for a common origin. Nat Chem Biol 5(4):244–250
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Propheter, D.C., Hsu, KL., Mahal, L.K. (2011). Recombinant Lectin Microarrays for Glycomic Analysis. In: Wu, C. (eds) Protein Microarray for Disease Analysis. Methods in Molecular Biology, vol 723. Humana Press. https://doi.org/10.1007/978-1-61779-043-0_6
Download citation
DOI: https://doi.org/10.1007/978-1-61779-043-0_6
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-042-3
Online ISBN: 978-1-61779-043-0
eBook Packages: Springer Protocols