Abstract
Sensors based on surface plasmon resonance have demonstrated, over the last 2 decades, to be an effective method of studying biomolecular interactions without the need for labeling. Recently, it has been adapted to high-throughput use for imaging microarray binding in real time. This provides a promising platform – a label-free protein microarray system – for the study of disease. In this example, antibody microarrays are used to efficiently profile the secretion of proteins from a cell line exposed to varying concentrations of a toxic compound.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Smith EA, Wanat MJ, Cheng Y, Barreira SVP, Frutos AG, Corn RM (2001) Formation, spectroscopic characterization, and application of sulfhydryl-terminated alkanethiol monolayers for the chemical attachment of DNA onto gold surfaces. Langmuir 17(8):2502–2507
Shumaker-Parry JS, Aebersold R, Campbell CT (2004) Parallel, quantitative measurement of protein binding to a 120-element double-stranded DNA array in real time using surface plasmon resonance microscopy. Anal Chem 76(7):2071–2082
Kyo M, Usui-Aoki K, Koga H (2005) Label-free detection of proteins in crude cell lysate with antibody arrays by a surface plasmon resonance imaging technique. Anal Chem 77(22):7115–7121
Usui-Aoki K, Shimada K, Nagano M, Kawai M, Koga H (2005) A novel approach to protein expression profiling using antibody microarrays combined with surface plasmon resonance technology. Proteomics 5(9):2396–2401
Lausted C, Hu Z, Hood L (2008) Quantitative serum proteomics from surface plasmon resonance imaging. Mol Cell Proteomics 7(12):2464–2474
de Boer A, Hokke C, Deelder A, Wuhrer M (2008) Serum antibody screening by surface plasmon resonance using a natural glycan microarray. Glycoconj J 25(1):75–84
Lokate AMC, Beusink JB, Besselink GAJ, Pruijn GJM, Schasfoort RBM (2007) Biomolecular Interaction Monitoring of Autoantibodies by Scanning Surface Plasmon Resonance Microarray Imaging. J Am Chem Soc 129(45):14013–14018
Karamanska R, Clarke J, Blixt O, MacRae J, Zhang J, Crocker P, Laurent N, Wright A, Flitsch S, Russell D, Field R (2008) Surface plasmon resonance imaging for real-time, label-free analysis of protein interactions with carbohydrate microarrays. Glycoconj J 25(1):69–74
Wassaf D, Kuang G, Kopacz K, Wu Q-L, Nguyen Q, Toews M, Cosic J, Jacques J, Wiltshire S, Lambert J, Pazmany CC, Hogan S, Ladner RC, Nixon AE, Sexton DJ (2006) High-throughput affinity ranking of antibodies using surface plasmon resonance microarrays. Anal Biochem 351(2):241–253
Peelen D, Kodoyianni V, Lee J, Zheng T, Shortreed MR, Smith LM (2006) Specific Capture of Mammalian Cells by Cell Surface Receptor Binding to Ligand Immobilized on Gold Thin Films. J Proteome Res 5(7):1580–1585
Suraniti E, Sollier E, Calemczuk R, Livache T, Marche PN, Villiers MB, Roupioz Y (2007) Real-time detection of lymphocytes binding on an antibody chip using SPR imaging. Lab Chip 7(9):1206–1208
Masubuchi Y (2006) Metabolic and non-metabolic factors determining troglitazone hepatotoxicity: a review. Drug Metab Pharmacokinet 21(5):347–356
Myszka DG, Morton TA (1998) CLAMP: a biosensor kinetic data analysis program. Trends Biochem Sci 23(4):149–150
Dudoit S, Gentleman RC, Quackenbush J (2003) Open source software for the analysis of microarray data. Biotechniques Suppl 45–51
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Lausted, C., Hu, Z., Hood, L. (2011). Label-Free Detection with Surface Plasmon Resonance Imaging. In: Wu, C. (eds) Protein Microarray for Disease Analysis. Methods in Molecular Biology, vol 723. Humana Press. https://doi.org/10.1007/978-1-61779-043-0_20
Download citation
DOI: https://doi.org/10.1007/978-1-61779-043-0_20
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-042-3
Online ISBN: 978-1-61779-043-0
eBook Packages: Springer Protocols