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Förster Resonance Energy Transfer Methods for Quantification of Protein–Protein Interactions on Microarrays

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Protein Microarray for Disease Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 723))

Abstract

Methods based on Förster (or fluorescence) resonance energy transfer (FRET) are widely used in various areas of bioanalysis and molecular biology, such as fluorescence microscopy, quantitative real-time polymerase chain reaction (PCR), immunoassays, or enzyme activity assays, just to name a few. In the last years, these techniques were successfully implemented to multiplex biomolecular screening on microarrays. In this review, some fundamental considerations and practical approaches are outlined and it is demonstrated how this very sensitive (and distance-dependent) method can be utilized for microarray-based high-throughput screening (HTS) with a focus on protein microarrays. The advantages and also the demands of this dual-label technique in miniaturized multiplexed formats are discussed with respect to its potential readout modes, such as intensity, dual wavelength, and time-resolved FRET detection.

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Authors and Affiliations

  1. Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg, Germany

    Michael Schäferling

  2. Institute of Analytical Chemistry, University of Leipzig, Leipzig, Germany

    Stefan Nagl

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  1. Michael Schäferling
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  2. Stefan Nagl
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Correspondence to Michael Schäferling .

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Editors and Affiliations

  1. Cancer Vaccine Center, Department of Medical Oncology, Dana-Farber Cancer Institute, Avenue Louis Pasteur 77, Boston, 02115, Massachusetts, USA

    Catherine J. Wu

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Schäferling, M., Nagl, S. (2011). Förster Resonance Energy Transfer Methods for Quantification of Protein–Protein Interactions on Microarrays. In: Wu, C. (eds) Protein Microarray for Disease Analysis. Methods in Molecular Biology, vol 723. Humana Press. https://doi.org/10.1007/978-1-61779-043-0_19

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  • DOI: https://doi.org/10.1007/978-1-61779-043-0_19

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  • Print ISBN: 978-1-61779-042-3

  • Online ISBN: 978-1-61779-043-0

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