Abstract
At the current rate of technological progress, high-throughput sequencing of nucleic acids has become a commodity. These techniques are perfectly suitable for viral small RNAs sequencing and contribute to the understanding of many aspects of virus biology in the context of host–pathogen interaction. However, the generation of high quality data is still an issue and the preparation of small RNAs libraries that accurately reflect the viral siRNAs in the sample remains a challenge. In this chapter we describe how to clone and sequence libraries of viral small RNAs from infected insect samples (mosquito, drosophilidae, insect-derived cell lines).
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Acknowledgments
The authors would like to thank Christophe Antoniewski for insight and discussion into deep sequencing, Marco Vignuzzi for comments on the manuscript, Nicolas Vodovar for helping with figures, and Ronald van Rij for being such a great and patient editor. This work was financially supported by the Agence Nationale de la Recherche (ANR-09-JCJC-0045-01) and the European Research Council (FP7/2007-2013 ERC 242703) to MCS.
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© 2011 Humana Press
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Gausson, V., Saleh, MC. (2011). Viral Small RNA Cloning and Sequencing. In: van Rij, R. (eds) Antiviral RNAi. Methods in Molecular Biology, vol 721. Humana Press. https://doi.org/10.1007/978-1-61779-037-9_6
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DOI: https://doi.org/10.1007/978-1-61779-037-9_6
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