Abstract
Polyamine blockade of inwardly rectifying potassium (Kir) channels underlies their steep voltage-Âdependence observed in native cells. The structural determinants of polyamine blockade and the structure-activity profile of endogenous polyamines requires specialized methodology for characterizing polyamine interactions with Kir channels. Recent identification and growing interest in the structure and function of prokaryotic Kir channels (KirBacs) has driven the development of new techniques for measuring ion channel activity. Several methods for measuring polyamine interactions with prokaryotic and eukaryotic Kir channels are discussed.
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References
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Kurata, H.T., Cheng, W.W.L., Nichols, C.G. (2011). Polyamine Block of Inwardly Rectifying Potassium Channels. In: Pegg, A., Casero, Jr., R. (eds) Polyamines. Methods in Molecular Biology, vol 720. Humana Press. https://doi.org/10.1007/978-1-61779-034-8_6
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DOI: https://doi.org/10.1007/978-1-61779-034-8_6
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