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Polyamines pp 67-79 | Cite as

Posttranscriptional Regulation of Gene Expression in Epithelial Cells by Polyamines

  • Lan Xiao
  • Jian-Ying WangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 720)

Abstract

In addition to regulating gene transcription, polyamines also potently modulate gene expression posttranscriptionally. Posttranscriptional gene regulation, which includes processes such as mRNA transport, turnover, and translation, involves specific mRNA sequences (cis-element) that interact with transacting factors such as RNA-binding proteins (RBPs) and microRNAs. U- or AU-rich elements (ARE) are the best characterized cis-acting sequences located in the 3’-untranslated regions of many labile mRNAs. Several RBPs, including AUF1, BRF1, TTP, and KSRP, promote ARE-mRNA decay through the recruitment of the ARE-bearing mRNA to sites of mRNA degradation, whereas RBPs such as HuR, HuB, HuC, and HuD stabilize target mRNAs and stimulate their translation. HuR is one of the best-studied RBPs and has emerged as a key regulator of posttranscriptional control of gene expression and its activity is tightly regulated by cellular polyamines. Ribonucleoprotein immunoprecipitation assays and biotin pull-down assays are two major methods used extensively in experiments investigating the roles and mechanisms of cellular polyamines in the posttranscriptional regulation and are described in detail in this chapter.

Key words

RNA-binding proteins, mRNA stability, and translation Posttranscriptional regulation Ribonucleoprotein 3’-Untranslated regions 

Notes

Acknowledgments

This work was supported by a Merit Review Grant (to J-Y Wang) from the Department of Veterans Affairs and by NIH Grants DK-57819, DK-61972, DK-68491 (to J-Y Wang). J-Y. Wang is a Research Career Scientist, Medical Research Service, US Department of Veterans Affairs.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Baltimore Veterans Affairs Medical CenterBaltimoreUSA

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