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Polyamines pp 505-518 | Cite as

Polyamine Analysis by LC-MS

  • Merja R. HäkkinenEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 720)

Abstract

This chapter describes a protocol to analyze polyamines without any derivatization steps utilizing LC-MS/MS. Polyamines are separated by reversed phase LC prior MS analysis using heptafluorobutyric acid as MS compatible volatile ion-pairing agent, and selective and sensitive MS detection is performed using MS/MS in selected reaction monitoring mode.

Key words

LC-MS/MS Method development Validation Polyamines Polyamine analogs Selected reaction monitoring 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Biosciences, Laboratory of Chemistry, Biocenter KuopioUniversity of KuopioKuopioFinland

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