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Polyamines pp 463-474 | Cite as

Fluorescent Substrates for Polyamine Catabolic Enzymes and Transport

  • Koichi Takao
  • Akira ShirahataEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 720)

Abstract

The most widely used methods for measuring polyamine enzyme activities are radioisotope methods that measure the radioactivity of compounds produced from radiolabeled substrate by the enzyme reaction. Several fluorescent polyamines have been developed for the measurement of the polyamine transport system (PTS) or transglutaminase. Although fluorophores in the fluorescent polyamines may affect the affinity of the polyamine moiety to the enzyme protein, the assays that use fluorescent substrate are sensitive and simple for common laboratory usage.

In this chapter, the uses of dansyl polyamines with a simple high-performance liquid chromatography system for the measurement of the PTS and polyamine catabolic enzymes including spermidine/spermine N1-acetyltransferase and N1-acetylpolyamine oxidase are described.

Key words

Dansyl polyamine Spermidine/spermine-N1-acetyltransferase (SSAT) N1-Acetylpolyamine oxidase (APAO) Polyamine transport system (PTS) Polyamine High-performance liquid ­chromatography (HPLC) 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Laboratory of Cellular Physiology, Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmaceutical SciencesJosai UniversitySakadoJapan
  2. 2.Department of Clinical Dietetics and Human Nutrition, Laboratory of Cellular PhysiologyJosai UniversitySakadoJapan

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