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Polyamines pp 51-65 | Cite as

Characterization of Genes for Polyamine Modulon

  • Kazuei IgarashiEmail author
  • Keiko Kashiwagi
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 720)

Abstract

Polyamines are essential for normal cell growth and exist mainly as RNA-polyamine complexes in cells. Thus, effects of polyamines on protein synthesis have been studied. It was found that several kinds of protein synthesis, which are important for cell growth, were enhanced by polyamines at the level of translation. We proposed that a group of genes whose expression is enhanced by polyamines at the level of translation be referred to as a “polyamine modulon.” In Escherichia coli, most members of the polyamine modulon thus far identified were transcription factors. These transcription factors enhanced the synthesis of several kinds of mRNA and tRNA, and also rRNA. In this way, polyamines enhanced growth of E. coli. We also succeeded in identifying three kinds of “polyamine modulon” in mammalian cells. One of the mechanisms of polyamine stimulation at the molecular level was due to the stabilization of the bulged-out region of double-stranded RNA in mRNA. The procedures used to identify components of the polyamine modulon are described in this chapter.

Key words

Polyamine modulon Protein synthesis Polyamine-RNA interaction Cell growth Cell viability Spermidine 

Notes

Acknowledgments

We are grateful to Drs. K. Williams and A. J. Michael for critical reading of the manuscript prior to submission. This study was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology, Japan.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Graduate School of Pharmaceutical SciencesChiba UniversityChibaJapan
  2. 2.Amine Pharma Research InstituteInnovation Plaza at Chiba UniversityChibaJapan
  3. 3.Faculty of PharmacyChiba Institute of ScienceChibaJapan

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