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Polyamines pp 379-394 | Cite as

Spermidine/Spermine-N1-Acetyltransferase in Kidney Ischemia Reperfusion Injury

  • Kamyar Zahedi
  • Manoocher Soleimani
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 720)

Abstract

Ischemic reperfusion injuries such as acute renal failure, acute liver failure, stroke, and myocardial infarction are prevalent causes of morbidity and mortality. Kidney ischemic reperfusion injury is the leading cause of acute renal failure and dysfunction of transplanted kidneys. Although significant progress has been made in deciphering the factors that contribute to ischemic reperfusion injury, treatment options for these injuries remain scant. Identifying the molecules that contribute to ischemic reperfusion injury and can be therapeutically targeted will lead to development of new approaches for the treatment of such injuries. The expression of spermidine/spermine-N1-acetyltransferase increases in the kidneys subjected to ischemic reperfusion injury. Furthermore, inactivation of the spermidine/spermine-N1-acetyltransferase gene reduces the severity of kidney damage after ischemic reperfusion injury. Enhanced expression of spermidine/spermine-N1-acetyltransferase in cultured cells leads to DNA damage, cell cycle arrest, and disruption of cell matrix interactions. The aforementioned observations strongly suggest that enhanced polyamine back conversion plays an important role in the mediation of tissue damage in renal Ischemic reperfusion injury.

Key words

Ischemic reperfusion injury Acute renal failure Spermidine/spermine-N1-acetyltransferase Polyamine back conversion 

Notes

Acknowledgements

The authors would like to thank Dr. Debora L. Kramer, Ms. Paula Diegelman, and Ms. Sharon L. Barone for their help with this manuscript. This work was supported by National Institutes of Health Grant RO1DK62809, Veterans Administration Merrit Review Award, and funds from the Center on Genetics of Transport at the University of Cincinnati.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Kamyar Zahedi
    • 1
  • Manoocher Soleimani
    • 1
  1. 1.Department of Surgery, Division of Nephrology and HypertensionUniversity of Cincinnati College of MedicineCincinnatiUSA

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