Procedures to Evaluate the Importance of Dietary Polyamines
Polyamines not only play vital physiological functions including modulating transcription and translation of genetic material, cell proliferation and growth, ion channel regulation and cell signaling, but have also been cited in the pathogenesis of diseases. Many plant and animal sources used as food contain high amounts of polyamines. Knowledge of the content of polyamines in food as a source of these growth factors is therefore critical. A 2-step perchloric acid precipitation method to obtain acid soluble extracts from food that are subsequently taken through a dansylation process to produce dansyl polyamine derivatives for HPLC measurement is described. Examples are provided to illustrate mathematical correction factors
Key wordsPolyamine Food Diet Dansylation Perchloric acid
We thank Gary Cameron (IMS core facilities) for invaluable support with HPLC analysis.
- 12.Milovic V, Odera G, Murphy GM, Dowling RH (1997) Jejunal putrescine absorption and the ‘pharmacokinetics’/biotransformation of ingested putrescine in humans. Gut 41:A62Google Scholar