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Polyamines pp 183-194 | Cite as

Characterization, Assay, and Substrate Specificity of Plant Polyamine Oxidases

  • Panagiotis N. Moschou
  • Kalliopi A. Roubelakis-AngelakisEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 720)

Abstract

Polyamine oxidation is the main catabolic process of polyamines. This process is crucial because not only it participates in the regulation of the endogenous titers of polyamines but also it generates hydrogen peroxide, which can act as a signaling molecule. The recent identification of polyamine oxidases that differ in substrate specificity and mode of action in plants necessitates the use of additional techniques for their characterization based on the determination of the end-product. Herein, we describe the most widely used techniques as well as new techniques that can be used for analysis of the newly identified polyamine oxidases.

Key words

Diamine oxidases Polyamine oxidases Oxidation Polyamine back-conversion Interconversion Hydrogen peroxide Flavin adenine dinucleotide Peroxisomes 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Panagiotis N. Moschou
    • 1
  • Kalliopi A. Roubelakis-Angelakis
    • 1
    Email author
  1. 1.Department of BiologyUniversity of CreteHeraklionGreece

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