Abstract
The in situ expression levels and subcellular localization of molecules involved in signal transduction using specific antibodies can be useful for prognosis and diagnosis of human diseases such as cancer. In addition, it has the potential to be helpful in monitoring biologic response to targeted therapies. The increasing availability of such antibodies makes these studies feasible. However, compared to typical immunohistochemical stains in which stabile molecules such as cytokeratins are targeted, additional validation may be required for signal transduction immunohistochemistry.
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The authors acknowledge Lisa Durkin for her technical expertise in immunostaining.
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Bodo, J., Hsi, E.D. (2011). Selection and Validation of Antibodies for Signal Transduction Immunohistochemistry. In: Kalyuzhny, A. (eds) Signal Transduction Immunohistochemistry. Methods in Molecular Biology, vol 717. Humana Press. https://doi.org/10.1007/978-1-61779-024-9_2
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DOI: https://doi.org/10.1007/978-1-61779-024-9_2
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