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Identifying Specific Trans-Factors of RNA Editing in Plant Mitochondria by Multiplex Single Base Extension Typing

  • Mizuki TakenakaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 718)

Abstract

The multiplex single base extension SNP-typing procedure outlined here can be employed to screen large numbers of plants for mutations in nuclear genes that affect mitochondrial RNA editing. The high ­sensitivity of this method allows high-throughput analysis of individual plants altered in RNA editing at given sites in total cellular cDNA from pooled RNA preparations of up to 50 green plants. The method can be used for large-scale screening for mutations in genes encoding trans-factors for specific RNA ­editing sites. Several nuclear encoded genes involved in RNA editing at specific sites in mitochondria of Arabidopsis thaliana have been identified by this approach.

Key words

RNA editing Plant mitochondria Mutant screening SNP typing 

Notes

Acknowledgements

I thank Dagmar Pruchner for excellent experimental help. I am very grateful to Walther Vogel, Christiane Maier and Bärbel Weber in the Institut für Humangenetik, Universität Ulm, for the use of the automated sequencing equipment and materials and their very constructive and helpful discussions throughout. This work was supported by grants from the DFG.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Molekulare BotanikUniversität UlmUlmGermany

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