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Mouse and Other Rodent Models of C to U RNA Editing

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RNA and DNA Editing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 718))

Abstract

Substitutional RNA editing represents an important posttranscriptional enzymatic pathway for increasing genetic plasticity by permitting production of different translation products from a single genomically encoded template. One of the best-characterized examples in mammals is C to U deamination of the nuclear apolipoprotein B (apoB) mRNA. ApoB mRNA undergoes a single, site-specific cytidine deamination event yielding an edited transcript that results in tissue-specific translation of two distinct isoforms, referred to as apoB100 and apoB48. Tissue- and site-specific cytidine deamination of apoB mRNA is mediated by an incompletely characterized holoenzyme containing a minimal core complex consisting of an RNA-specific cytidine deaminase, Apobec-1 and a requisite cofactor, apobec-1 complementation factor (ACF). The underlying biochemical and genetic mechanisms regulating tissue-specific apoB mRNA editing have been accelerated through development and characterization of physiological rodent models as well as knockout and transgenic animal strains.

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Acknowledgments

Work cited in this review was supported by grants from the National Institutes of Health (HL-38180, DK-56260, DK-52574) to NOD. The authors are deeply grateful to Susan Kennedy and Jianyang Luo for assistance with the murine models quoted in this review.

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Authors and Affiliations

  1. Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA

    Valerie Blanc & Nicholas O. Davidson

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  1. Valerie Blanc
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  2. Nicholas O. Davidson
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Correspondence to Nicholas O. Davidson .

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Editors and Affiliations

  1. School of Medicine, Dept. Microbiology & Molecular Genetics, University of California, Irvine, Irvine, 92697, California, USA

    Ruslan Aphasizhev

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Blanc, V., Davidson, N.O. (2011). Mouse and Other Rodent Models of C to U RNA Editing. In: Aphasizhev, R. (eds) RNA and DNA Editing. Methods in Molecular Biology, vol 718. Humana Press. https://doi.org/10.1007/978-1-61779-018-8_7

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  • DOI: https://doi.org/10.1007/978-1-61779-018-8_7

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-017-1

  • Online ISBN: 978-1-61779-018-8

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