Post-transcriptional Modification of RNAs by Artificial Box H/ACA and Box C/D RNPs

  • Chao Huang
  • John Karijolich
  • Yi-Tao YuEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 718)


RNA-guided RNA 2′-O-methylation and pseudouridylation are naturally occurring processes, in which guide RNAs specifically direct modifications to rRNAs or spliceosomal snRNAs in the nucleus of eukaryotic cells. Modifications can profoundly alter the properties of an RNA, thus influencing the contributions of the RNA to the cellular process in which it participates. Recently, it has been shown that, by expressing artificial guide RNAs (derived from naturally occurring guide RNAs), modifications can also be specifically introduced into other RNAs, thus offering an opportunity to study RNAs in vivo. Here, we present strategies for constructing guide RNAs and manipulating RNA modifications in the nucleus.

Key words

snoRNA 2′-O-methylation Pseudouridylation snRNA rRNA mRNA Telomerase RNA Box C/D RNA Box H/ACA RNA 



We would like to thank the members of the Yu Laboratory for discussion and inspiration. Our work was supported by grant GM62937 (to Yi-Tao Yu) from the National Institute of Health. J.K. was supported by a NIH Institutional Ruth L. Kirschstein National Research Service Award GM068411.


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© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Biochemistry and BiophysicsUniversity of Rochester Medical CenterRochesterUSA

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