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Complementation of Mutants in Plant Mitochondrial RNA Editing by Protoplast Transfection

  • Mizuki TakenakaEmail author
  • Anja Zehrmann
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 718)

Abstract

A crucial and often decisive test of a nuclear gene being involved in a given process is the complementation of mutants. Restoring the wild type phenotype by the wild type gene introduced into the mutant is a major piece of evidence for the function of this gene. We have developed a rapid and reliable method to complement protoplasts from plants with mutations in mitochondrial RNA editing with the respective wild type genes. The method furthermore allows testing the functionality of modified protein sequences without the need to make and grow transgenic plants, which is very time-consuming. We successfully employed this method for several nuclear-encoded genes involved in RNA editing at specific sites in mitochondria of Arabidopsis thaliana.

Key words

RNA editing Plant mitochondria Mutant screening SNP typing 

Notes

Acknowledgments

We thank Dagmar Pruchner for excellent experimental help. This work was supported by a grant from the DFG to M.T.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Molekulare BotanikUniversität UlmUlmGermany

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