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Oligosaccharide Mass Profiling (OLIMP) of Cell Wall Polysaccharides by MALDI-TOF/MS

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The Plant Cell Wall

Part of the book series: Methods in Molecular Biology ((MIMB,volume 715))

Abstract

In today’s field of plant cell wall research, insights into the structure of wall components are obtained using many different techniques, ranging from spectroscopic and microscopic to chemical and biochemical. In this chapter, we describe one method: oligosaccharide mass profiling (OLIMP). Using OLIMP, we can harness the selective power of a specific wall hydrolase together with the speed and sensitivity of mass spectrometry to provide highly reproducible structural and compositional information about the wall molecule of interest.

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Acknowledgements

The authors would like to thank Kirk Schorr (Novozymes) for providing the enzymes XEG and PME. Karen Bird, DOE-Plant Research Lab, Michigan State University, is thanked for editing the text.

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Authors and Affiliations

  1. Energy Biosciences Institute, University of California, Berkeley, CA, USA

    Markus Günl, Florian Kraemer & Markus Pauly

Authors
  1. Markus Günl
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  2. Florian Kraemer
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  3. Markus Pauly
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Corresponding author

Correspondence to Markus Pauly .

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Editors and Affiliations

  1. School of Natural Sciences, Dept. of Botany & Plant Science, National University of Ireland, Galway, University Road, Galway, Ireland

    Zoë A. Popper

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Günl, M., Kraemer, F., Pauly, M. (2011). Oligosaccharide Mass Profiling (OLIMP) of Cell Wall Polysaccharides by MALDI-TOF/MS. In: Popper, Z. (eds) The Plant Cell Wall. Methods in Molecular Biology, vol 715. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-008-9_3

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  • DOI: https://doi.org/10.1007/978-1-61779-008-9_3

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-007-2

  • Online ISBN: 978-1-61779-008-9

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