Abstract
Arabinogalactan proteins are a diverse group of plant cell wall-associated proteoglycans. While structural and molecular genetic analyses have contributed to the emerging improved understanding of the wide-range of biological processes in which AGPs are implicated; the ability to detect, localise, and quantify them is fundamentally important. This chapter describes two commonly used methods, histological staining and radial gel diffusion, both of which utilise the ability of Yariv reagent to bind to AGPs.
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Acknowledgements
Quentin Coster, a visiting MSc student from the UniversitƩ Catholique de Louvain, Belgium, and Tanya Slattery, an undergraduate project student in Botany and Plant Science at NUI Galway, are thanked for their technical assistance.
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Popper, Z.A. (2011). Extraction and Detection of Arabinogalactan Proteins. In: Popper, Z. (eds) The Plant Cell Wall. Methods in Molecular Biology, vol 715. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-008-9_17
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DOI: https://doi.org/10.1007/978-1-61779-008-9_17
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