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RNA Detection and Visualization pp 407–422Cite as

RIP: An mRNA Localization Technique

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 714))

Abstract

A detailed understanding of post-transcriptional gene expression is necessary to correlate the different elements involved in the many levels of RNA–protein interactions that are needed to coordinate the cellular biomolecular machinery. The profile of mRNA, a major component of this machinery, can be examined after isolation from specific RNA-binding proteins (RBPs). RIP-Chip or ribonomic profiling is a versatilein vivo technique that has been widely used to study post-transcriptional gene regulation and the localization of mRNA. Here we elaborately detail the methodology for mRNA isolation using RBP immunoprecipitation (RIP) as a primary approach. Specific antibodies are used to target RBPs, which are then used to capture the associated mRNA.

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Acknowledgments

We wish to thank the members of the Tenenbaum Lab and specifically Arthur Beauregard for helpful editorial comments. This work was supported in part by NIH/NHGRI Grant 5U01HG004571 to SAT.

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Authors and Affiliations

  1. Department of Nanobioscience, University at Albany, Albany, NY, USA

    Scott A. Tenenbaum

Authors
  1. Sabarinath Jayaseelan
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  2. Francis Doyle
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  3. Salvatore Currenti
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  4. Scott A. Tenenbaum
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Corresponding author

Correspondence to Scott A. Tenenbaum .

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Editors and Affiliations

  1. , Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel

    Jeffrey E. Gerst

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Jayaseelan, S., Doyle, F., Currenti, S., Tenenbaum, S.A. (2011). RIP: An mRNA Localization Technique. In: Gerst, J. (eds) RNA Detection and Visualization. Methods in Molecular Biology, vol 714. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-005-8_25

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  • DOI: https://doi.org/10.1007/978-1-61779-005-8_25

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  • Publisher Name: Humana Press, Totowa, NJ

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