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Chickpea Hybridization Using In Vitro Techniques

  • Nalini Mallikarjuna
  • Fred J. Muehlbauer
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 710)

Abstract

Tissue culture techniques play an important role in the utilization of wild Cicer species for the improvement­ of cultivated chickpea. Utilization of wild Cicer species has become essential as a series of evolutionary bottlenecks have narrowed the genetic base of chickpea, thus making it susceptible to a range of diseases and pests. Crosses with wild Cicer can broaden its genetic base and introduce useful traits. Except for two wild species, none of the other Cicer species are cross-compatible. To use a range of Cicer species for the improvement of chickpea, embryo rescue and tissue culture techniques are necessary. The success of the cross with incompatible species depended on a range of techniques including the application of growth regulators to pollinated pistils and saving aborting embryos in vitro. Further, the chances of successful transfer of hybrid shoots to soil are greater if the hybrid shoots are grafted to chickpea stocks.

Key words

Chickpea Cicer species Cleft graft Embryo rescue Growth regulators Incompatibility 

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Copyright information

© Humana Press 2011

Authors and Affiliations

  • Nalini Mallikarjuna
    • 1
  • Fred J. Muehlbauer
    • 2
  1. 1.International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)PatancheruIndia
  2. 2.Agricultural Research Service, United States Department of AgricultureWashington State UniversityPullmanUSA

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