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Metabolic Profiling pp 229–246Cite as

Capillary Electrophoresis–Electrospray Ionization-Mass Spectrometry (CE–ESI-MS)-Based Metabolomics

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 708))

Abstract

Metabolomics is a rapidly emerging field of functional genomics research whose aim is the comprehensive analysis of low molecular weight metabolites in a biological sample. Capillary electrophoresis–electrospray ionization-mass spectrometry (CE–ESI-MS) represents a promising hyphenated microseparation platform in metabolomics, since a majority of primary metabolites are intrinsically polar. CE–ESI-MS offers a convenient format for the separation of complex mixtures of cationic, anionic, and/or zwitterionic metabolites, as well as their isobaric/isomeric ions without complicated sample handling. Moreover, online sample preconcentration with desalting is readily integrated during separation prior to ionization, where the migration behavior and ionization response of metabolites can be predicted based on their fundamental physicochemical properties. Herein, we describe recent developments in CE–ESI-MS with emphasis on practical protocols necessary for realizing reliable analyses as applied to targeted metabolite profiling and untargeted metabolomic studies in various biological samples.

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Acknowledgments

The author wishes to acknowledge funding support from National Science and Engineering Research Council of Canada, Premier’s Research Excellence Award, as well as a Japan Society for Promotion of Science – Invitation Fellowship.

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Authors and Affiliations

  1. Department of Chemistry and Chemical Biology, McMaster University, Hamilton, ON, Canada

    Philip Britz-McKibbin

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  1. Philip Britz-McKibbin
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Correspondence to Philip Britz-McKibbin .

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Editors and Affiliations

  1. Fundamental & Computational Sciences Dir, Pacific Northwest National Laboratory, Battelle Boulevard 902, Richland, 99352, Washington, USA

    Thomas O. Metz

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Britz-McKibbin, P. (2011). Capillary Electrophoresis–Electrospray Ionization-Mass Spectrometry (CE–ESI-MS)-Based Metabolomics. In: Metz, T. (eds) Metabolic Profiling. Methods in Molecular Biology, vol 708. Humana Press. https://doi.org/10.1007/978-1-61737-985-7_14

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  • DOI: https://doi.org/10.1007/978-1-61737-985-7_14

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61737-984-0

  • Online ISBN: 978-1-61737-985-7

  • eBook Packages: Springer Protocols

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