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Golden Retriever Muscular Dystrophy (GRMD): Developing and Maintaining a Colony and Physiological Functional Measurements

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Muscle Gene Therapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 709))

Abstract

Studies of canine models of Duchenne muscular dystrophy (DMD) provide insight regarding disease pathogenesis and treatment efficacy. To take maximal advantage, colonies of affected dogs must be maintained and outcome parameters developed. In this chapter, we review our 25 years of experience with the golden retriever muscular dystrophy (GRMD) model. Key challenges in colony development (breeding, neonatal death, and the risk of inbreeding) and representative functional measurements (tibiotarsal joint angle and torque force; and eccentric contraction decrement) are discussed.

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References

  1. Bulfield, G., Siller, W.G., Wight, P. A. L., and Moore, K. J. (1984) X chromosome-linked muscular dystrophy (mdx) in the mouse. Proc Natl Acad Sci USA 81, 1189–1192.

    Article  PubMed  CAS  Google Scholar 

  2. Gillis, J. M. (1999) Understanding dystrophinopathies: an inventory of the structural and functional consequences of the absence of dystrophin in muscles of the mdx mouse. J Muscle Res Cell Motil 20, 605–625.

    Article  PubMed  CAS  Google Scholar 

  3. Kornegay, J. N., Tuler, S. M., Miller, D. M., and Levesque, D. C. (1988) Muscular dystrophy in a litter of golden retriever dogs. Muscle Nerve 11, 1056–1064.

    Article  PubMed  CAS  Google Scholar 

  4. Cooper, B. J., Winand, W. N., Stedman, H., Valentine B. A., Hoffman, E. P., Kunkel, L. M., et al. (1988) The homologue of the Duchenne locus is defective in X-linked muscular dystrophy of dogs. Nature 334, 154–156.

    Article  PubMed  CAS  Google Scholar 

  5. Schatzberg, S. J., Olby, N. J., Breen, M., Anderson, L. V. B., Langford, C. F., Dickens H. F., et al. (1999) Molecular analysis of a spontaneous dystrophin “knockout” dog. Neuromuscul Disord 9, 289–295.

    Article  PubMed  CAS  Google Scholar 

  6. Jones, B. R., Brennan, S., Mooney, C. T., Callanan, J. J., McAllister, H., Guo, L. T. et al. (2004) Muscular dystrophy with truncated dystrophin in a family of Japanese Spitz dogs. J Neurol Sci 217, 143–149.

    Article  PubMed  CAS  Google Scholar 

  7. Baltzer, W. I., Calise, D. V., Levine, J. M., Shelton, G. D., Edwards, J. F., and Steiner, J. M. (2007) Dystrophin-deficient muscular dystrophy in a Weimaraner. J Am Anim Hosp Assoc 43, 227–232.

    PubMed  Google Scholar 

  8. Gaschen, F. P., Hoffman, E. P., Gorospe, J. R., Uhl, E. W., Senior, D. F., Cardinet, G. H. 3rd, et al. (1992) Dystrophin deficiency causes lethal muscle hypertrophy in cats. J Neurol Sci 110, 149–159.

    Article  PubMed  CAS  Google Scholar 

  9. Winand, N. J., Edwards, M., Pradhan, D., Berian, C. A., and Cooper, B. J. (1994) Deletion of the dystrophin muscle promoter in feline muscular dystrophy. Neuromuscul Disord 4, 433–445.

    Article  PubMed  CAS  Google Scholar 

  10. Sharp, N. J. H., Kornegay, J. N., Van Camp, S. D., Herbstreith, M. H., Secore, S. L., Kettle, S., et al. (1991) An error in dystrophin mRNA processing in golden retriever muscular dystrophy, an animal homologue of Duchenne muscular dystrophy. Genomics 13, 115–121.

    Article  Google Scholar 

  11. Vignos, P. J., Jr., Spencer, G. E., Jr, and Archibald, J. C. (1963) Management of ­muscular dystrophy of childhood. JAMA 184, 89–96.

    PubMed  Google Scholar 

  12. Brooke, M. H., Fenichel, G. M., Griggs, R. C., Mendell, J. R., Moxley, R., Miller, J. P., et al. (1983) Clinical Investigation in Duchenne dystrophy: 2. Determination of the “power” of therapeutic trials based on the natural history. Muscle Nerve 6, 91–103.

    Article  PubMed  CAS  Google Scholar 

  13. Siegel, I. M., Miller, J. E., and Ray, R. D. (1968) Subcutaneous lower limb tenotomy in the treatment of pseudohypertrophic muscular dystrophy. J Bone Joint Surg 50A, 1437–1443.

    Google Scholar 

  14. Kornegay, J. N., Sharp, N. J. H., Schueler, R. O., and Betts, C. W. (1994) Tarsal joint contracture in dogs with golden retriever muscular dystrophy. Lab Anim Sci 44, 331–333.

    PubMed  CAS  Google Scholar 

  15. Kornegay, J. N., Sharp, N. J. H., Bogan, D. J., Van Camp, S. D., Metcalf, J. R., and Schueler, R. O. (1994) Contraction tension and kinetics of the peroneus longus muscle in golden retriever muscular dystrophy. J Neurol Sci 123, 100–107.

    Article  PubMed  CAS  Google Scholar 

  16. Kornegay, J. N., Bogan, D. J., Bogan, J. R., Childers, M. K., Cundiff, D. D., Petroski, G. F., et al. (1999) Contraction force generated by tarsal joint flexion and extension in dogs with golden retriever muscular dystrophy. J Neurol Sci 166, 115–121.

    Article  PubMed  CAS  Google Scholar 

  17. Childers, M. K., Okamura, C. S., Bogan, D. J., Bogan, J. R., Petroski, G. F., McDonald K., et al. (2002) Eccentric contraction injury in dystrophic canine muscle. Arch Phys Med Rehabil 83, 1572–1578.

    Article  PubMed  Google Scholar 

  18. Liu, J. M. K., Okamura, C. S., Bogan, D. J., Bogan, J. R, Childers, M. K., and Kornegay, J. N. (2004) Effects of prednisone in canine muscular dystrophy. Muscle Nerve 30, 767–773.

    Article  PubMed  Google Scholar 

  19. Jaegger, G., Marcellin-Little, D. J., and Levine, D. (2002) Reliability of goniometry in Labrador retrievers. Am J Vet Res 63, 979–986.

    Article  PubMed  Google Scholar 

  20. Nicholson, H. L., Osmotherly, P. G., Smith, B.A., and McGowan, C. M. (2007) Determinants of passive hip range of motion in adult Greyhounds. Aust Vet J 85, 217–221.

    Article  PubMed  CAS  Google Scholar 

  21. Edwards, R. H. T., Jones, D. A., Newham, D. J., and Chapman, S. J. (1984) Role of mechanical damage in the pathogenesis of proximal myopathy in man. Lancet 1, 548–552.

    Article  PubMed  CAS  Google Scholar 

  22. Dangain, J. and Vrbova, G. (1984) Muscle development in mdx mutant mice. Muscle Nerve 7, 700–704.

    Article  PubMed  CAS  Google Scholar 

  23. Valentine, B. A. and Cooper, B. J. (1991) Canine X-linked muscular dystrophy: selective involvement of muscles in neonatal dogs. Neuromuscul Disord 1, 31–38.

    Article  PubMed  CAS  Google Scholar 

  24. Moens, P., Baatsen, P. H. W. W., and Marechal, G. (1993) Increased susceptibility of EDL muscles from mdx mice to damage induced by contractions with stretch. J Mus Res Cell Motil 14, 446–451.

    Article  CAS  Google Scholar 

  25. Thomassen, R., Sanson, G., Krogenæs, A., Fougnerb, J. A., Andersen Berg, A., and Farstad, W. (2006) Artificial insemination with frozen semen in dogs: a retrospective study of 10 years using a non-surgical approach. Theriogenology 66, 1645–1650.

    Article  PubMed  CAS  Google Scholar 

  26. Kelley, R. L. (2001) Factors influencing canine reproduction and nutritional management of the pregnant bitch. Canine Reproduction and Nutritional Health – Tufts Animal Expo, pp 9–14.

    Google Scholar 

  27. Kornegay, J. N., Cundiff, D. D., Bogan, D. J., Bogan, J. R., and Okamura, C. S. (2003) The cranial sartorius muscle undergoes true hypertrophy in dogs with golden retriever muscular dystrophy. Neuromuscul Disord 13, 493–500.

    Article  PubMed  Google Scholar 

  28. Salimena, M. C., Lagrota-Candido, J., and Quírico-Santos, T. (2004) Gender dimorphism influences extracellular matrix expression and regeneration of muscular tissue in mdx dystrophic mice. Histochem Cell Biol 122, 535–544.

    Google Scholar 

  29. Valentine, B. A., Cooper, B. J., de Lahunta, A, O’Quinn, R., and Blue, J. T. (1988) Canine X-linked muscular dystrophy. An animal model of Duchenne muscular dystrophy: clinical studies. J Neurol Sci 88, 69–81.

    Article  PubMed  CAS  Google Scholar 

  30. Gordon, A. M., Huxley, A. F., and Julian, F. J. (1966) The variation in isometric tension with sarcomere length in vertebrate muscle fibres. J Physiol 184, 170–192.

    PubMed  CAS  Google Scholar 

  31. Hoy, M. G., Zajac, F. E., and Gordon, M. E. (1990) A musculoskeletal model of the human lower extremity: the effect of muscle, tendon, and moment arm on the moment-angle relationship of musculotendon actuators at the hip, knee, and ankle. J Biomechanics 23, 157–169.

    Article  CAS  Google Scholar 

  32. Lieber, R. L. and Boakes, J. L. (1988) Sarcomere length and joint kinematics during torque production in frog hindlimb. Am J Physiol 254, C759–C768.

    PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine and The Gene Therapy Center, School of Medicine, University of North Carolina-Chapel Hill, Chapel Hill, NC, USA

    Joe N. Kornegay, Janet R. Bogan & Daniel J. Bogan

  2. Department of Neurology and Wake Forest Institute for Regenerative Medicine, School of Medicine, Wake Forest University, Winston-Salem, NC, USA

    Martin K. Childers

  3. Department of Human Nutrition, Foods, and Exercise, College of Agriculture and Life Sciences, Virginia Tech University, Blacksburg, USA

    Robert W. Grange

Authors
  1. Joe N. Kornegay
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  2. Janet R. Bogan
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  3. Daniel J. Bogan
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  4. Martin K. Childers
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  5. Robert W. Grange
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Corresponding author

Correspondence to Joe N. Kornegay .

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Editors and Affiliations

  1. , Dept. of Molecular Microbiology, University of Missouri, Hospital Dr. 1, Columbia, 65212, Missouri, USA

    Dongsheng Duan

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Kornegay, J.N., Bogan, J.R., Bogan, D.J., Childers, M.K., Grange, R.W. (2011). Golden Retriever Muscular Dystrophy (GRMD): Developing and Maintaining a Colony and Physiological Functional Measurements. In: Duan, D. (eds) Muscle Gene Therapy. Methods in Molecular Biology, vol 709. Humana Press. https://doi.org/10.1007/978-1-61737-982-6_7

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  • DOI: https://doi.org/10.1007/978-1-61737-982-6_7

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61737-981-9

  • Online ISBN: 978-1-61737-982-6

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