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Muscle Gene Therapy pp 91–104Cite as

Phenotyping Cardiac Gene Therapy in Mice

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 709))

Abstract

Heart disease is the leading health problem of industrialized countries. The development of gene therapies tailored towards the heart has grown exponentially over the past decade. Murine models of heart diseases have played a pivotal role in testing novel cardiac gene therapy approaches. Unfortunately, the small body size and rapid heart rate of mice present a great challenge to heart function evaluation. Here we outline the commonly used cardiac phenotyping methods of treadmill exercise regimen, full 12-lead electrocardiographic assay and left ventricular catheterization hemodynamic assay. Application of these protocols will allow critical testing of gene therapy efficacy in mouse models of heart diseases.

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Acknowledgments

This work is supported by grants from the National Institutes of Health HL-91883 and the Muscular Dystrophy Association (DD). We thank Mr. Nate Marschalk and Dr. Dejia Li for helpful discussion on treadmill function assay.

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Authors and Affiliations

  1. Department of Molecular Microbiology and Immunology, School of Medicine, The University of Missouri, One Hospital Drive, Columbia, MO, USA

    Brian Bostick

  2. Department of Molecular Microbiology and Immunology, School of Medicine, The University of Missouri, One Hospital Drive, Columbia, USA

    Yongping Yue

  3. Department of Molecular Microbiology and Immunology, School of Medicine, The University of Missouri, 1 Hospital Drive, M610, Columbia, MO, USA

    Dongsheng Duan

Authors
  1. Brian Bostick
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  2. Yongping Yue
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  3. Dongsheng Duan
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Corresponding author

Correspondence to Dongsheng Duan .

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Editors and Affiliations

  1. , Dept. of Molecular Microbiology, University of Missouri, Hospital Dr. 1, Columbia, 65212, Missouri, USA

    Dongsheng Duan

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Bostick, B., Yue, Y., Duan, D. (2011). Phenotyping Cardiac Gene Therapy in Mice. In: Duan, D. (eds) Muscle Gene Therapy. Methods in Molecular Biology, vol 709. Humana Press. https://doi.org/10.1007/978-1-61737-982-6_6

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  • DOI: https://doi.org/10.1007/978-1-61737-982-6_6

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61737-981-9

  • Online ISBN: 978-1-61737-982-6

  • eBook Packages: Springer Protocols

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