Abstract
Analysis of the human genome sequence has identified thousands of putative genes with unknown function; therefore, a new tool allowing for rapid identification of gene functions is needed. Reverse transfection microarray technology, which turns a DNA microarray into a cell-based microarray, has emerged for simultaneously studying the function of many genes. Since the initial demonstration in 2001, many variations have surfaced, making the technology more versatile for a broad range of applications. We have developed a protocol to make ready-to-transfect DNA microarrays in a 96-well microplate for co-transfection of two plasmids into HEK293T cells. This cell-based microarray in a microplate may be used for screening hundreds of analytes against multiple protein targets in parallel, providing a powerful tool for functional genomics and drug discovery.
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Acknowledgments
We would like to thank Brian L. Webb for his pivotal role in developing the technology and Janie Causer for her excellent support in printing DNA microarrays.
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Soni, M., Lai, F. (2011). Cell-Based Co-transfection Microarrays for Use with HEK293T Cells on a Poly d-Lysine-Coated Polystyrene Microplate. In: Palmer, E. (eds) Cell-Based Microarrays. Methods in Molecular Biology, vol 706. Humana Press. https://doi.org/10.1007/978-1-61737-970-3_2
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DOI: https://doi.org/10.1007/978-1-61737-970-3_2
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