Abstract
Protein–protein interaction is one of the most pivotal roles of proteins in living organisms. Association/dissociation of proteins reflects responses to intrinsic or extrinsic perturbations of signaling pathways, involved in gene expression, cell division, cell differentiation, and apoptosis. For further understanding of the biological processes, it is important to monitor protein–protein interactions in model organisms. In particular, Escherichia coli-based methods are suitable to assess large libraries of proteins. Many of these proteins cannot be used in yeast due to toxicity or poor expression. Herein we describe a general method based on an intein-mediated protein reconstitution system (PRS) to detect protein–protein interactions in bacterial cells. The PRS-based approach requires no other agents including enzymes, substrates, and ATP. Another advantage is that matured green fluorescent protein (GFP) accumulates in a targeted cell till degraded. These allow highly sensitive screening of protein–protein interactions.
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Acknowledgment
This work was financially supported by Core Research for Evolutional Science and Technology (CREST) of Japan Science and Technology Agency (JST) and Japan Society for the Promotion of Science (JSPS).
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Kanno, A., Ozawa, T., Umezawa, Y. (2011). Detection of Protein–Protein Interactions in Bacteria by GFP-Fragment Reconstitution. In: Evans, Jr., T., Xu, MQ. (eds) Heterologous Gene Expression in E.coli. Methods in Molecular Biology, vol 705. Humana Press. https://doi.org/10.1007/978-1-61737-967-3_15
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DOI: https://doi.org/10.1007/978-1-61737-967-3_15
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