Abstract
Automatic flow-based systems have been applied to assay scavenging capacity against reactive oxygen and nitrogen species, providing analytical tools which can cope with different types and large number of samples. In the present chapter, a flow injection analysis procedure is described for the assessment of peroxynitrite scavenging. A sequential injection analysis procedure is also described for determining the scavenging capacity against the nitric oxide radical. For both systems, reaction between putative antioxidants and the reactive species of nitrogen takes place inside the flow conduits before addition of luminol and further detection of remaining reactive species by chemiluminescence.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ruzicka, J., Hansen, E. H. (1975) Flow injection analyses. 1. New concept of fast continuous-flow analysis. Anal Chim Acta 78, 145–157.
Hansen, E. H., Norgaard, L., Pedersen, M. (1991) Optimization of flow-injection systems for determination of substrates by means of enzyme amplification reactions and chemiluminescence detection. Talanta 38, 275–282.
Ruzicka, J., Marshall, G. D. (1990) Sequential injection – a new concept for chemical sensors, process analysis and laboratory assays. Anal Chim Acta 237, 329–343.
Segundo, M. A., Magalhães, L. M. (2006) Multisyringe flow injection analysis: state-of-the-art and perspectives. Anal Sci 22, 3–8.
Magalhães, L. M., Santos, M., Segundo, M. A., Reis, S., Lima, J. L. F. C. (2009) Flow injection based methods for fast screening of antioxidant capacity. Talanta 77, 1559–1566.
Magalhães, L. M., Lucio, M., Segundo, M. A., Reis, S., Lima, J. L. F. C. (2009) Automatic flow injection based methodologies for determination of scavenging capacity against biologically relevant reactive species of oxygen and nitrogen. Talanta 78, 1219–1226.
Magalhães, L. M., Ribeiro, J. P. N., Segundo, M. A., Reis, S., Lima, J. L. F. C. (2009) Multi-syringe flow-injection systems improve antioxidant assessment. Trend Anal Chem 28, 952–960.
Sariahmetoglu, M., Wheatley, R. A., Cakici, I., Kanzik, I., Townshend, A. (2003) Flow injection analysis for monitoring antioxidant effects on luminol chemiluminescence of reactive oxygen species. Anal Lett 36, 749–765.
Beckman, J. S., Chen, J., Ischiropoulos, H., Crow, J. P. (1996) Oxidative chemistry of peroxynitrite. Method Enzymol 269, 229–240.
Yao, D. C., Vlessidis, A. G., Evmiridis, N. P. (2001) On-line monitoring of nitric oxide complexed with porphyrine-bearing biochemical materials by using flow injection with chemiluminescence detection. Anal Chim Acta 435, 273–280.
Yao, D. C., Vlessidis, A. G., Evmiridis, N. P., Evangelou, A., Karkabounas, S., Tsampalas, S. (2002) Luminol chemiluminescence reaction: a new method for monitoring nitric oxide in vivo. Anal Chim Acta 458, 281–289.
Yao, D. C., Vlessidis, A. G., Evmiridis, N. P., Siminelakis, S., Dimitra, M. (2004) Possible mechanism for nitric oxide and oxidative stress induced pathophysiological variance in acute myocardial infarction development – a study by a flow injection-chemiluminescence method. Anal Chim Acta 505, 115–123.
Wang, J. N., Lu, M. Q., Yang, F. Z., Zhang, X. R., Baeyens, W. R. G., Campaña, A. M. G. (2001) Microdialysis with on-line chemiluminescence detection for the study of nitric oxide release in rat brain following traumatic injury. Anal Chim Acta 428, 173–181.
Miyamoto, A., Nakamura, K., Kishikawa, N., Ohba, Y., Nakashima, K., Kuroda, N. (2007) Quasi-simultaneous determination of antioxidative activities against superoxide anion and nitric oxide by a combination of sequential injection analysis and flow injection analysis with chemiluminescence detection. Anal Bioanal Chem 388, 1809–1814.
Hughes, M. N., Nicklin, H. G. (1968) Chemistry of pernitrites. I. Kinetics of decomposition of pernitrous acid. J Chem Soc A 2, 450–452.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Segundo, M.A., Magalhães, L.M., Ribeiro, J.P., Lúcio, M., Reis, S. (2011). Determination of the Scavenging Capacity Against Reactive Nitrogen Species by Automatic Flow Injection-Based Methodologies. In: McCarthy, H., Coulter, J. (eds) Nitric Oxide. Methods in Molecular Biology, vol 704. Humana Press. https://doi.org/10.1007/978-1-61737-964-2_8
Download citation
DOI: https://doi.org/10.1007/978-1-61737-964-2_8
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61737-963-5
Online ISBN: 978-1-61737-964-2
eBook Packages: Springer Protocols