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Nitric Oxide pp 91-104 | Cite as

Determination of the Scavenging Capacity Against Reactive Nitrogen Species by Automatic Flow Injection-Based Methodologies

  • Marcela A. SegundoEmail author
  • Luís M. Magalhães
  • Joana P.N. Ribeiro
  • Marlene Lúcio
  • Salette Reis
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 704)

Abstract

Automatic flow-based systems have been applied to assay scavenging capacity against reactive oxygen and nitrogen species, providing analytical tools which can cope with different types and large number of samples. In the present chapter, a flow injection analysis procedure is described for the assessment of peroxynitrite scavenging. A sequential injection analysis procedure is also described for determining the scavenging capacity against the nitric oxide radical. For both systems, reaction between putative antioxidants and the reactive species of nitrogen takes place inside the flow conduits before addition of luminol and further detection of remaining reactive species by chemiluminescence.

Key words

Flow injection analysis sequential injection analysis scavenging capacity reactive species of nitrogen nitric oxide peroxynitrite chemiluminescence automation 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Marcela A. Segundo
    • 1
    Email author
  • Luís M. Magalhães
    • 1
  • Joana P.N. Ribeiro
    • 1
  • Marlene Lúcio
    • 1
  • Salette Reis
    • 1
  1. 1.REQUIMTE, Departamento de Química-Física, Faculdade de FarmáciaUniversidade do PortoPortoPortugal

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