Ionizing Radiation-Induced DNA Strand Breaks and γ-H2AX Foci in Cells Exposed to Nitric Oxide
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A number of studies have demonstrated that nitric oxide enhances radiosensitivity of anoxic and hypoxic cells in vitro and in vivo, and some evidence points to a role for DNA damage and repair in this phenomenon. We have recently observed that nitric oxide enhances the formation of DNA single- and double-strand breaks following ionising irradiation, measured by the alkaline comet assay and immunofluorescence microscopy for γ-H2AX .
Key wordsNitric oxide DNA strand break γ-H2AX single cell gel electrophoresis ionising radiation
The methods described here were employed in a recent Cancer Research UK-funded study at the Gray Cancer Institute under Peter Wardman’s leadership, with support from Mick Woodcock, Lisa Folkes and Peter Johnston.
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