Abstract
A number of studies have demonstrated that nitric oxide enhances radiosensitivity of anoxic and hypoxic cells in vitro and in vivo, and some evidence points to a role for DNA damage and repair in this phenomenon. We have recently observed that nitric oxide enhances the formation of DNA single- and double-strand breaks following ionising irradiation, measured by the alkaline comet assay and immunofluorescence microscopy for γ-H2AX .
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Acknowledgements
The methods described here were employed in a recent Cancer Research UK-funded study at the Gray Cancer Institute under Peter Wardman’s leadership, with support from Mick Woodcock, Lisa Folkes and Peter Johnston.
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Rothkamm, K., Burdak-Rothkamm, S. (2011). Ionizing Radiation-Induced DNA Strand Breaks and γ-H2AX Foci in Cells Exposed to Nitric Oxide. In: McCarthy, H., Coulter, J. (eds) Nitric Oxide. Methods in Molecular Biology, vol 704. Humana Press. https://doi.org/10.1007/978-1-61737-964-2_2
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DOI: https://doi.org/10.1007/978-1-61737-964-2_2
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