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Methods for the Purification and Characterization of Human Adipose-Derived Stem Cells

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Adipose-Derived Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 702))

Abstract

Peripheral adipose tissue contains a population of clonogenic precursor cells referred to as adipose-derived stem cells (ASC) that retain the capacity to differentiate into multiple cell types including osteoblasts, adipocytes, chondrocytes, myocytes, and neuronal cells following ex vivo expansion. Recent studies have demonstrated that ASC are most likely derived from a perivascular niche within highly vascularised fat tissue, analogous to different mesenchymal cell populations identified in other tissues throughout the body. The following chapter describes techniques to prospectively isolate clonogenic ASC from adult human adipose tissue using antibodies directed against perivascular markers and methods to immunophenotypically characterize their ex vivo expanded progeny.

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Acknowledgments

The authors wish to acknowledge the technical assistance of Ms. Sharon Paton and Ms. Romana Borowicz.

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Authors and Affiliations

  1. Mesenchymal Stem Cell Group, Department of Haematology, Institute of Medical and Veterinary Science/Hanson Institute and Centre for Stem Cell Research, University of Adelaide, Adelaide, SA, Australia

    Stan Gronthos

  2. Myeloma Research Laboratory, Department of Haematology, Institute of Medical and Veterinary Science/Centre for Cancer Biology and the Centre for Stem Cell Research, University of Adelaide, Adelaide, SA, Australia

    Andrew C. W. Zannettino

Authors
  1. Stan Gronthos
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  2. Andrew C. W. Zannettino
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Corresponding author

Correspondence to Stan Gronthos .

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Editors and Affiliations

  1. , Stem Cell Biology, Pennington Biomedical Research Center, Perkins Rd. 6400, Baton Rouge, 70808, Louisiana, USA

    Jeffrey M. Gimble

  2. School of Medicine, Department of Pharmacology, Tulane University, Tulane Ave. 1430, New Orleans, 70112-2699, Louisiana, USA

    Bruce A. Bunnell

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Gronthos, S., Zannettino, A.C.W. (2011). Methods for the Purification and Characterization of Human Adipose-Derived Stem Cells. In: Gimble, J., Bunnell, B. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 702. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61737-960-4_9

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  • DOI: https://doi.org/10.1007/978-1-61737-960-4_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61737-959-8

  • Online ISBN: 978-1-61737-960-4

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